ArticleThe Journal of cell biology1994
Reconstitution of native-type noncrystalline lens fiber gap junctions from isolated hemichannels.
Article in The Journal of cell biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
12 citing papers in PubMed, 51 citations in OpenAlex.
- Lipids intercalate and mediate multi-channel assemblies of connexin-46/50 gap junctions.bioRxiv : the preprint server for biology · 2026Article
- Connexin-46/50 in a dynamic lipid environment resolved by CryoEM at 1.9 Å.Nature communications · 2020Article
- The Aquaporin Splice Variant NbXIP1;1α Is Permeable to Boric Acid and Is Phosphorylated in the N-terminal Domain.Frontiers in plant science · 2016Article
- Identification of the WNK-SPAK/OSR1 signaling pathway in rodent and human lenses.Investigative ophthalmology & visual science · 2014Article
- Projection structure of a N-terminal deletion mutant of connexin 26 channel with decreased central pore density.Cell communication & adhesion · 2008Article
- Change in permeant size selectivity by phosphorylation of connexin 43 gap-junctional hemichannels by PKC.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- The supramolecular architecture of junctional microdomains in native lens membranes.EMBO reports · 2007Article
- Heteromerization of innexin gap junction proteins regulates epithelial tissue organization in Drosophila.Molecular biology of the cell · 2006Article
- MP20, the second most abundant lens membrane protein and member of the tetraspanin superfamily, joins the list of ligands of galectin-3.BMC cell biology · 2001Article
- Connexins/connexons. Cell-free expression.Methods in molecular biology (Clifton, N.J.) · 2001Article
- Cell-free synthesis and assembly of connexins into functional gap junction membrane channels.The EMBO journal · 1997Article
- Heteromeric connexons in lens gap junction channels.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Gap junctions contain numerous channels that are clustered in apposed membrane patches of adjacent cells. These cell-to-cell channels are formed by pairing of two hemichannels or connexons, and are also referred to as connexon pairs. We have investigated various detergents for their ability to separately solubilize hemichannels or connexon pairs from isolated ovine lens fiber membranes. The solubilized preparations were reconstituted with lipids with the aim to reassemble native-type gap junctions and to provide a model system for the characterization of the molecular interactions involved in this process. While small gap junction structures were obtained under a variety of conditions, large native-type gap junctions were assembled using a novel two-step procedure: in the first step, hemichannels that had been solubilized with octylpolyoxyethylene formed connexon pairs by dialysis against n-decyl-beta-D-maltopyranoside. In the second step, connexon pairs were reconstituted with phosphatidylcholines by dialysis against buffer containing Mg2+. This way, double-layered gap junctions with diameter < or = 300 nm were obtained. Up to several hundred channels were packed in a noncrystalline arrangement, giving these reconstituted gap junctions an appearance that was indistinguishable from that of the gap junctions in the lens fiber membranes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.