ArticleEuropean journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology1994
Routine laboratory diagnosis of continuous ambulatory peritoneal dialysis peritonitis using centrifugation/lysis and saponin-containing media.
Article in European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
3 citing papers in PubMed.
- Detecting bacterial growth in continuous ambulatory peritoneal dialysis effluent using two culture methods.The Korean journal of internal medicine · 2010Article
- Improved detection of bacterial growth in continuous ambulatory peritoneal dialysis effluent by use of BacT/Alert FAN bottles.Journal of clinical microbiology · 1997Article
- Use of prototype automated blood culture system and gas-liquid chromatography for the analysis of continuous ambulatory peritoneal dialysis associated infection.Journal of clinical pathology · 1997Article
Corrections and comments
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Authors and funding
1 author.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study examines the application in a routine laboratory of a method for concentrating and lysing leucocytes in continuous ambulatory peritoneal dialysis (CAPD) effluent to diagnose peritonitis. Between November 1986 and April 1990 pathogens from 42 of 82 (51%) CAPD specimens from patients with acute peritonitis failed to grow on horse blood agar (HBA) after direct inoculation. Recovery rates of pathogens were 74% (6 of 82) from a solid medium containing saponin and 89% (73 of 82) after lysing the leucocytes before inoculating HBA. Microbial sequestration in leucocytes was demonstrated in 73% (60 of 82) of specimens, while pathogens from 11% (9 of 82) were grown only from a liquid medium. Thirteen of 17 specimens with < 500 leucocytes/mm3 grew on all three solid media. Gram stain microscopy revealed organisms in 51% (41 of 81) of specimens. The method, using apparatus and media prepared for everyday examination of specimens, is sensitive, specific and yields single colonies of most species within 24 h.
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Registered trials
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