ArticleThe Journal of cell biology1994
Transformation of Drosophila melanogaster with the wild-type myosin heavy-chain gene: rescue of mutant phenotypes and analysis of defects caused by overexpression.
Article in The Journal of cell biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed, 49 citations in OpenAlex.
- The Drosophila CG1674 gene encodes a synaptopodin 2-like related protein that localizes to the Z-disc and is required for normal flight muscle development and function.Developmental dynamics : an official publication of the American Association of Anatomists · 2021Article
- Five Alternative Myosin Converter Domains Influence Muscle Power, Stretch Activation, and Kinetics.Biophysical journal · 2018Article
- Overexpression of miRNA-9 Generates Muscle Hypercontraction Through Translational Repression of Troponin-T inG3 (Bethesda, Md.) · 2017Article
- Functional redundancy and nonredundancy between two Troponin C isoforms inMolecular biology of the cell · 2017Article
- A Restrictive Cardiomyopathy Mutation in an Invariant Proline at the Myosin Head/Rod Junction Enhances Head Flexibility and Function, Yielding Muscle Defects in Drosophila.Journal of molecular biology · 2016Article
- An undergraduate laboratory class using CRISPR/Cas9 technology to mutate drosophila genes.Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology · 2016Article
- Article
- An embryonic myosin converter domain influences Drosophila indirect flight muscle stretch activation, power generation and flight.The Journal of experimental biology · 2014Article
- Expression of the inclusion body myopathy 3 mutation in Drosophila depresses myosin function and stability and recapitulates muscle inclusions and weakness.Molecular biology of the cell · 2012Article
- Drosophila UNC-45 accumulates in embryonic blastoderm and in muscles, and is essential for muscle myosin stability.Journal of cell science · 2011Article
- CF2 represses Actin 88F gene expression and maintains filament balance during indirect flight muscle development in Drosophila.PloS one · 2010Article
- The mechanical properties of Drosophila jump muscle expressing wild-type and embryonic Myosin isoforms.Biophysical journal · 2010Article
- Crossveinless and the TGFbeta pathway regulate fiber number in the Drosophila adult jump muscle.Development (Cambridge, England) · 2009Article
- Alternative relay domains of Drosophila melanogaster myosin differentially affect ATPase activity, in vitro motility, myofibril structure and muscle function.Journal of molecular biology · 2008Article
- Myosin transducer mutations differentially affect motor function, myofibril structure, and the performance of skeletal and cardiac muscles.Molecular biology of the cell · 2008Article
- mef2 activity levels differentially affect gene expression during Drosophila muscle development.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- A variable domain near the ATP-binding site in Drosophila muscle myosin is part of the communication pathway between the nucleotide and actin-binding sites.Journal of molecular biology · 2007Article
- Site directed mutagenesis of Drosophila flightin disrupts phosphorylation and impairs flight muscle structure and mechanics.Journal of muscle research and cell motility · 2007Article
- Overexpression of troponin T in Drosophila muscles causes a decrease in the levels of thin-filament proteins.The Biochemical journal · 2005Article
- Suppression of muscle hypercontraction by mutations in the myosin heavy chain gene of Drosophila melanogaster.Genetics · 2003Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
We have transformed Drosophila melanogaster with a genomic construct containing the entire wild-type myosin heavy-chain gene, Mhc, together with approximately 9 kb of flanking DNA on each side. Three independent lines stably express myosin heavy-chain protein (MHC) at approximately wild-type levels. The MHC produced is functional since it rescues the mutant phenotypes of a number of different Mhc alleles: the amorphic allele Mhc1, the indirect flight muscle and jump muscle-specific amorphic allele Mhc10, and the hypomorphic allele Mhc2. We show that the Mhc2 mutation is due to the insertion of a transposable element in an intron of Mhc. Since a reduction in MHC in the indirect flight muscles alters the myosin/actin protein ratio and results in myofibrillar defects, we determined the effects of an increase in the effective copy number of Mhc. The presence of four copies of Mhc results in overabundance of the protein and a flightless phenotype. Electron microscopy reveals concomitant defects in the indirect flight muscles, with excess thick filaments at the periphery of the myofibrils. Further increases in copy number are lethal. These results demonstrate the usefulness and potential of the transgenic system to study myosin function in Drosophila. They also show that overexpression of wild-type protein in muscle may disrupt the function of not only the indirect flight but also other muscles of the organism.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.