ArticleProceedings of the National Academy of Sciences of the United States of America1994
Transient transfection of the enteric parasite Entamoeba histolytica and expression of firefly luciferase.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 63 citations in OpenAlex.
- Work with me here: variations in genome content and emerging genetic tools in Entamoeba histolytica.Trends in parasitology · 2025Review
- Growth and Genetic Manipulation of Entamoeba histolytica.Current protocols · 2022Article
- Regulation of H2O2 stress-responsive genes through a novel transcription factor in the protozoan pathogen Entamoeba histolytica.The Journal of biological chemistry · 2013Article
- Regulation of gene expression in protozoa parasites.Journal of biomedicine & biotechnology · 2010Review
- The 29-kilodalton thiol-dependent peroxidase of Entamoeba histolytica is a factor involved in pathogenesis and survival of the parasite during oxidative stress.Eukaryotic cell · 2007Article
- Identification and characterization of a cyclooxygenase-like enzyme from Entamoeba histolytica.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Pathogenesis of intestinal amebiasis: from molecules to disease.Clinical microbiology reviews · 2000Review
- Control of ferredoxin and Gal/GalNAc lectin gene expression in Entamoeba histolytica by a cis-acting DNA sequence.Infection and immunity · 1998Article
- Transcription initiation is controlled by three core promoter elements in the hgl5 gene of the protozoan parasite Entamoeba histolytica.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Transient and selectable transformation of the parasitic protist Trichomonas vaginalis.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Transfection and continuous expression of heterologous genes in the protozoan parasite Entamoeba histolytica.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
- Virus-mediated expression of firefly luciferase in the parasitic protozoan Giardia lamblia.Molecular and cellular biology · 1995Article
- Transient transfection and expression of firefly luciferase in Giardia lamblia.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Development of DNA-mediated transfection in Entamoeba histolytica will facilitate basic research toward the control of this protozoan parasite. A transient transfection system was established by using the firefly luciferase gene ligated to the 5' and 3' flanking regions of the amebic hgl1 gene. The optimal construct tested encoded an hgl1-luciferase fusion protein and contained 1 kb of 5' flanking sequence with 16 bases of coding sequence from the hgl1 gene ligated in-frame to the luciferase start codon and 2.3 kb of 3' flanking sequence from hgl1 ligated 3' to the luciferase stop codon. Optimal electroporation conditions in strain HM-1:IMSS trophozoites when using this construct were 500 microF and 500 V/cm, which resulted in luciferase activity up to 5000-fold above background 9-12 hr after electroporation. Constructs that contained the luciferase gene without amebic flanking sequences or that contained a simian virus 40 promoter, enhancer, and polyadenylylation signal produced only background levels of luciferase activity. The ability to introduce and express genes in amebae will now permit a genetic analysis of the virulence of this organism, which remains a serious threat to world health.
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