ArticleProceedings of the National Academy of Sciences of the United States of America1994
Transfection and transient expression of chloramphenicol acetyltransferase gene in the protozoan parasite Entamoeba histolytica.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Work with me here: variations in genome content and emerging genetic tools in Entamoeba histolytica.Trends in parasitology · 2025Review
- Growth and Genetic Manipulation of Entamoeba histolytica.Current protocols · 2022Article
- Anaerobic peroxisomes in Entamoeba histolytica metabolize myo-inositol.PLoS pathogens · 2021Article
- Ubc7/Ube2g2 ortholog in Entamoeba histolytica: connection with the plasma membrane and phagocytosis.Parasitology research · 2018Article
- A Novel Heat Shock Element (HSE) inFrontiers in cellular and infection microbiology · 2017Article
- Article
- Multinucleation and Polykaryon Formation is Promoted by the EhPC4 Transcription Factor in Entamoeba histolytica.Scientific reports · 2016Article
- InMicrobial cell (Graz, Austria) · 2015Article
- The small GTPase EhRabB of Entamoeba histolytica is differentially expressed during phagocytosis.Parasitology research · 2013Article
- Regulation of gene expression in protozoa parasites.Journal of biomedicine & biotechnology · 2010Review
- Transient expression of red and yellow fluorescent protein vectors in HCT-8 cells infected with Cryptosporidium parvum.Parasitology research · 2009Article
- Promoter analysis of palindromic transcription units in the ribosomal DNA circle of Entamoeba histolytica.Eukaryotic cell · 2009Article
- Structural and functional analysis of the Entamoeba histolytica EhrabB gene promoter.BMC molecular biology · 2007Article
- The 29-kilodalton thiol-dependent peroxidase of Entamoeba histolytica is a factor involved in pathogenesis and survival of the parasite during oxidative stress.Eukaryotic cell · 2007Article
- Expression of the nfa1 gene cloned from pathogenic Naegleria fowleri in nonpathogenic N. gruberi enhances cytotoxicity against CHO target cells in vitro.Infection and immunity · 2005Article
- The intestinal protozoan parasite Entamoeba histolytica contains 20 cysteine protease genes, of which only a small subset is expressed during in vitro cultivation.Eukaryotic cell · 2003Article
- Article
- Pathogenesis of intestinal amebiasis: from molecules to disease.Clinical microbiology reviews · 2000Review
- Virulence and functions of myosin II are inhibited by overexpression of light meromyosin in Entamoeba histolytica.Molecular biology of the cell · 1998Article
- Control of ferredoxin and Gal/GalNAc lectin gene expression in Entamoeba histolytica by a cis-acting DNA sequence.Infection and immunity · 1998Article
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2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hybrid plasmids were constructed and used for successful transfection and transient expression of the chloramphenicol acetyltransferase (CAT) gene in the protozoan parasite Entamoeba histolytica. Transfection was performed by electroporation of the amebae in a potassium phosphate-based buffer under conditions of 3000 V/cm and 25 microF, resulting in a time constant of 0.4 ms. Expression of CAT activity was achieved with constructs in which the CAT coding region was flanked by untranslated upstream and downstream sequences of E. histolytica genes. Highest activity was detected after culturing transfected cells for 48 hr. Activity was found to be proportional to the amount of DNA transfected.
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