ArticleProceedings of the National Academy of Sciences of the United States of America1994
Renal epithelial cells rapidly bind and internalize calcium oxalate monohydrate crystals.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 144 citations in OpenAlex.
- The Application of Nanomaterials in Kidney Stone Disease: Emerging Strategies for Early Diagnosis, Targeted Therapy, and Prevention.International journal of nanomedicine · 2026Review
- Early Detection and Monitoring of Nephrolithiasis: The Potential of Electrochemical Sensors.Sensors (Basel, Switzerland) · 2025Review
- Calcium Phosphate-Induced Injury in Kidney Tubular Cells-Supersaturated Solution vs Preformed Crystals.Journal of biochemical and molecular toxicology · 2025Article
- The upregulation of lamin A/C as a compensatory mechanism during tight junction disruption in renal tubular cells mediated by calcium oxalate crystals.Current research in toxicology · 2024Article
- Article
- Cell Protection and Crystal Endocytosis Inhibition by SulfatedACS omega · 2023Article
- Antioxidant Activity ofBioinorganic chemistry and applications · 2023Article
- Induction of retinopathy by fibrillar oxalate assemblies.Communications chemistry · 2020Article
- Comparison of the adhesion and endocytosis of calcium oxalate dihydrate to HK-2 cells before and after repair byScience and technology of advanced materials · 2019Article
- The Long Pentraxin PTX3 Is an Endogenous Inhibitor of Hyperoxaluria-Related Nephrocalcinosis and Chronic Kidney Disease.Frontiers in immunology · 2018Article
- Kidney Stone Disease: An Update on Current Concepts.Advances in urology · 2018Review
- Systematic evaluation for effects of urine pH on calcium oxalate crystallization, crystal-cell adhesion and internalization into renal tubular cells.Scientific reports · 2017Article
- Hyperoxaluria Requires TNF Receptors to Initiate Crystal Adhesion and Kidney Stone Disease.Journal of the American Society of Nephrology : JASN · 2017Article
- Surface heat shock protein 90 serves as a potential receptor for calcium oxalate crystal on apical membrane of renal tubular epithelial cells.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2016Article
- In vitro inhibition of calcium oxalate crystallization and crystal adherence to renal tubular epithelial cells by Terminalia arjuna.Urolithiasis · 2016Article
- Genetic modulation of nephrocalcinosis in mouse models of ectopic mineralization: the Abcc6(tm1Jfk) and Enpp1(asj) mutant mice.Laboratory investigation; a journal of technical methods and pathology · 2014Article
- Update on oxalate crystal disease.Current rheumatology reports · 2013Review
- Pathomechanisms of nephrolithiasis.Hippokratia · 2013Review
- Nephrolithiasis: molecular mechanism of renal stone formation and the critical role played by modulators.BioMed research international · 2013Review
- Aluminum citrate prevents renal injury from calcium oxalate crystal deposition.Journal of the American Society of Nephrology : JASN · 2012Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Renal tubular fluid is supersaturated with calcium and oxalate ions, which can nucleate to form crystals of calcium oxalate monohydrate (COM), the most abundant constituent of kidney stones. However, the mechanisms by which nascent crystals are retained in the nephron and then grow into kidney stones are unclear. An interaction of COM crystals with the surface of renal epithelial cells could be a critical initiating event in nephrolithiasis. To investigate this possibility we used cultures of monkey kidney epithelial cells (BSC-1 line) as a model system and found that [14C]COM crystals bound to the cell surface within seconds. Scanning electron microscopy revealed that crystals bind first to apical microvilli, which subsequently migrate over the crystalline surface. When visualized by transmission electron microscopy, intracellular crystals were located within vesicles. Cytoskeletal responses to crystal uptake were sought by immunofluorescence microscopy, which revealed concentration of F-actin at sites of crystal contact as well as a generalized reorganization of the intermediate filament network containing cytokeratin 8. Uptake of COM crystals did not adversely affect renal epithelial cell growth, and internalized crystals were apparently distributed to daughter cells during division. Rapid adherence of COM crystals to the apical surface of tubular epithelial cells could promote crystal retention in the kidney. Elucidation of factors that regulate this process may provide insight into the pathogenesis of nephrolithiasis.
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