ArticleProceedings of the National Academy of Sciences of the United States of America1994
Frog diazepam-binding inhibitor: peptide sequence, cDNA cloning, and expression in the brain.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 55 citations in OpenAlex.
- Cytoprotective and Neurotrophic Effects of Octadecaneuropeptide (ODN) inFrontiers in endocrinology · 2020Review
- TSPO: An Evolutionarily Conserved Protein with Elusive Functions.International journal of molecular sciences · 2018Review
- Endozepines.Advances in pharmacology (San Diego, Calif.) · 2015Review
- Endogenous positive allosteric modulation of GABA(A) receptors by diazepam binding inhibitor.Neuron · 2013Article
- Regulation of neurosteroid biosynthesis by neurotransmitters and neuropeptides.Frontiers in endocrinology · 2012Article
- The anorexigenic action of the octadecaneuropeptide (ODN) in goldfish is mediated through the MC4R- and subsequently the CRH receptor-signaling pathways.Journal of molecular neuroscience : MN · 2010Article
- Translocator protein 18 kDa (TSPO): molecular sensor of brain injury and repair.Pharmacology & therapeutics · 2008Review
- Acyl-CoA binding proteins; structural and functional conservation over 2000 MYA.Molecular and cellular biochemistry · 2007Article
- ACBP4 and ACBP5, novel Arabidopsis acyl-CoA-binding proteins with kelch motifs that bind oleoyl-CoA.Plant molecular biology · 2004Article
- Membrane localization of Arabidopsis acyl-CoA binding protein ACBP2.Plant molecular biology · 2003Article
- Overexpression of acyl-coA binding protein and its effects on the flux of free fatty acids in McA-RH 7777 cells.Lipids · 2001Article
- Depletion of acyl-coenzyme A-binding protein affects sphingolipid synthesis and causes vesicle accumulation and membrane defects in Saccharomyces cerevisiae.Molecular biology of the cell · 2001Article
- Single amino acid substitutions at the acyl-CoA-binding domain interrupt 14[C]palmitoyl-CoA binding of ACBP2, an Arabidopsis acyl-CoA-binding protein with ankyrin repeats.Plant molecular biology · 2000Article
- Arabidopsis cDNA encoding a membrane-associated protein with an acyl-CoA binding domain.Plant molecular biology · 1998Article
- Role of long-chain fatty acyl-CoA esters in the regulation of metabolism and in cell signalling.The Biochemical journal · 1997Review
- Occurrence of two somatostatin variants in the frog brain: characterization of the cDNAs, distribution of the mRNAs, and receptor-binding affinities of the peptides.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- Review
- Localization of 17beta-hydroxysteroid dehydrogenase and characterization of testosterone in the brain of the male frog.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- The characterization of two diazepam binding inhibitor (DBI) transcripts in humans.The Biochemical journal · 1995Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Three peptides derived from diazepam-binding inhibitor (DBI) were isolated in pure form from the brain of the frog Rana ridibunda. The primary structures of these peptides showed that they correspond to mammalian DBI-(1-39), DBI-(58-87), and DBI-(70-87). A set of degenerate primers, whose design was based on the amino acid sequence data, was used to screen a frog brain cDNA library. The cloned cDNA encodes an 87-amino acid polypeptide, which exhibits 68% similarity with porcine and bovine DBI. Frog DBI contains two paired basic amino acids (Lys-Lys) at positions 14-15 and 62-63 and a single cysteine within the biologically active region of the molecule. Northern blot analysis showed that DBI mRNA is expressed at a high level in the brain but is virtually absent in peripheral tissues. The distribution of DBI mRNA and DBI-like immunoreactivity in the frog brain was studied by in situ hybridization and immunocytochemistry. Both approaches revealed that the DBI gene is expressed in ependymal cells and circumventricular organs lining the ventricular cavity. Since amphibia diverged from mammals at least 250 million years ago, the data show that evolutionary pressure has acted to conserve the structure of DBI in the vertebrate phylum. The distribution of both DBI mRNA and DBI-like immunoreactivity indicates that DBI is selectively expressed in glial cells.
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