ArticleThe Histochemical journal1994
Quantification in immunohistochemistry: the measurement of the ratios of collagen types I and II.
Article in The Histochemical journal, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
5 citing papers in PubMed.
- Extracellular Matrix Protein Ratios in the Human Heart and Vessels: How to Distinguish Pathological From Physiological Changes?Frontiers in physiology · 2021Review
- Down-regulation of heat shock protein HSP90ab1 in radiation-damaged lung cells other than mast cells.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2014Article
- Comparison of different detection methods in quantitative microdensitometry.The American journal of pathology · 2001Article
- Enhancement of scleral macromolecular permeability with prostaglandins.Transactions of the American Ophthalmological Society · 2001Article
- Immunohistochemical video-microdensitometry of myocardial collagen type I and type III.The Histochemical journal · 1998Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Quantitative techniques in immunohistochemistry are needed, but they are rarely applied because of doubtful reproducibility. We have developed a method for the detection of collagen types I and III in situ. The method applied was a two-step immuno-alkaline phosphatase technique with visualization of the end-product with Fast Red. The staining intensity was measured with a microdensitometer and the results expressed as ratios. The method yielded results that were unaffected by variations in tissue section thickness but which were proportionally related to time and antigen concentrations. Leiomyoma tissue, with a ratio of collagen types I and III of approximately 1.0, was used to establish the appropriate dilutions of the antibodies, thus assuring identical optical densities. By having the leiomyoma tissue sections incubated together with the heart tissue specimens, leiomyoma tissue was also helpful in correcting deviations from the 1.0 ratio. Accurate measurements of collagen type I/III ratios in normal human heart specimens were obtained with the present quantitative immunohistochemical technique.
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