ArticleThe EMBO journal1994
A cell-specific enhancer far upstream of the mouse tyrosinase gene confers high level and copy number-related expression in transgenic mice.
Article in The EMBO journal, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed, 115 citations in OpenAlex.
- Boundary sequences flanking the mouse tyrosinase locus ensure faithful pattern of gene expression.Scientific reports · 2020Article
- Epigenetic mechanisms in health and disease: BCEC 2017.Epigenetics · 2018Article
- Upregulation ofBioMed research international · 2017Article
- Functional validation of mouse tyrosinase non-coding regulatory DNA elements by CRISPR-Cas9-mediated mutagenesis.Nucleic acids research · 2015Article
- Proinvasion metastasis drivers in early-stage melanoma are oncogenes.Cancer cell · 2011Article
- Promoters with cancer cell-specific activity for melanoma gene therapy.Acta naturae · 2011Article
- An insulator embedded in the chicken α-globin locus regulates chromatin domain configuration and differential gene expression.Nucleic acids research · 2011Article
- Analysis of adenovirus trans-complementation-mediated gene expression controlled by melanoma-specific TETP promoter in vitro.Virology journal · 2010Article
- Transcriptional effects of glucocorticoid receptors in the dentate gyrus increase anxiety-related behaviors.PloS one · 2009Article
- Article
- Cellular genetic tools to control oncolytic adenoviruses for virotherapy of cancer.Journal of molecular medicine (Berlin, Germany) · 2008Review
- Rescue of the albino phenotype by introducing a functional tyrosinase minigene into Kunming albino mice.World journal of gastroenterology · 2007Article
- A strategy to study tyrosinase transgenes in mouse melanocytes.BMC cell biology · 2005Article
- The Tyr (albino) locus of the laboratory mouse.Mammalian genome : official journal of the International Mammalian Genome Society · 2004Article
- A simple method to cure established tumors by inflammatory killing of normal cells.Nature biotechnology · 2004Article
- MIA (melanoma inhibitory activity) promoter mediated tissue-specific suicide gene therapy of malignant melanoma.Cancer gene therapy · 2004Article
- Article
- Melanocytes and pigmentation are affected in dopachrome tautomerase knockout mice.Molecular and cellular biology · 2004Article
- Functional dissection of the mouse tyrosinase locus control region identifies a new putative boundary activity.Nucleic acids research · 2003Article
- A novel attenuated replication-competent adenovirus for melanoma therapy.Gene therapy · 2003Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The tyrosinase gene encodes the key enzyme of melanin production and is tightly regulated during development. A yeast artificial chromosome covering the mouse tyrosinase gene has been shown to rescue completely the albino phenotype of recipient mouse strains, conferring copy number-dependent, position-independent expression. To investigate the presence of cis-acting regulatory elements responsible for the appropriate expression of the tyrosinase gene, DNase I hypersensitive site mapping was performed. A melanoma cell-specific DNase I hypersensitive site was identified at -12 kb upstream of the tyrosinase gene. Functional analysis of the corresponding cis-acting element in transgenic mice and transient transfection assays revealed properties of a strong cell-specific enhancer. RNA expression levels of the transgene correlate with copy number, which is reflected in coat colour and eye pigmentation of transgenic mice. Full enhancer activity in transient transfections is obtained with a minimal sequence of 200 bp. Protein binding analysis reveals the presence of a melanoma cell-specific complex which might contribute to the faithful expression of the tyrosinase gene.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.