ArticleThe Biochemical journal1994
Characterization of 14-3-3 proteins in adrenal chromaffin cells and demonstration of isoform-specific phospholipid binding.
Article in The Biochemical journal, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 60 citations in OpenAlex.
- From 1957 to Nowadays: A Brief History of Epigenetics.International journal of molecular sciences · 2020Review
- YWHA (14-3-3) protein isoforms and their interactions with CDC25B phosphatase in mouse oogenesis and oocyte maturation.BMC developmental biology · 2019Article
- Phosphorylation dependence and stoichiometry of the complex formed by tyrosine hydroxylase and 14-3-3γ.Molecular & cellular proteomics : MCP · 2014Article
- Components of the CtBP1/BARS-dependent fission machinery.Histochemistry and cell biology · 2013Review
- Expression of 14-3-3 protein isoforms in mouse oocytes, eggs and ovarian follicular development.BMC research notes · 2012Article
- The peripheral binding of 14-3-3γ to membranes involves isoform-specific histidine residues.PloS one · 2012Article
- A neurotoxic phospholipase A2 impairs yeast amphiphysin activity and reduces endocytosis.PloS one · 2012Article
- Implications for proteasome nuclear localization revealed by the structure of the nuclear proteasome tether protein Cut8.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Three-way interaction between 14-3-3 proteins, the N-terminal region of tyrosine hydroxylase, and negatively charged membranes.The Journal of biological chemistry · 2009Article
- Flow cytometry-assisted purification and proteomic analysis of the corticotropes dense-core secretory granules.Proteomics · 2008Article
- Arachidonic acid binds 14-3-3zeta, releases 14-3-3zeta from phosphorylated BAD and induces aggregation of 14-3-3zeta.Neurochemical research · 2008Article
- Proteomic screen in the simple metazoan Hydra identifies 14-3-3 binding proteins implicated in cellular metabolism, cytoskeletal organisation and Ca2+ signalling.BMC cell biology · 2007Article
- Unchanged survival rates of 14-3-3gamma knockout mice after inoculation with pathological prion protein.Molecular and cellular biology · 2005Article
- ADP-ribosylation of Rab5 by ExoS of Pseudomonas aeruginosa affects endocytosis.Infection and immunity · 2001Article
- 14-3-3tau associates with and activates the MEF2D transcription factor during muscle cell differentiation.Nucleic acids research · 2001Article
- Molecular evolution of the 14-3-3 protein family.Journal of molecular evolution · 1996Article
- Molecular organization and tissue-specific expression of an Arabidopsis 14-3-3 gene.The Plant cell · 1996Article
- Identification of 14-3-3 proteins in human platelets: effects of synthetic peptides on protein kinase C activation.The Biochemical journal · 1996Article
- Distinct effects of alpha-SNAP, 14-3-3 proteins, and calmodulin on priming and triggering of regulated exocytosis.The Journal of cell biology · 1995Article
- Post-translationally modified 14-3-3 isoforms and inhibition of protein kinase C.Molecular and cellular biochemistryArticle
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Isoform-specific antisera were used to examine which 14-3-3 isoforms were present in bovine adrenal chromaffin cells. The eta, tau and sigma isoforms were not detectable, and the epsilon isoform was present at only low levels. 14-3-3 isoforms were readily detected with antisera against the beta, gamma and zeta isoforms. The latter isoforms were found to leak from digitonin-permeabilized chromaffin cells, as expected for cytosolic proteins, but a proportion of each isoform was retained. In subcellular fractionation studies isoforms recognized by the beta and zeta antisera were found in the cytosol and Triton-insoluble cytoskeletal fractions, while the gamma isoform was found in cytosol and also in microsomal and chromaffin granule membrane fractions. The gamma 14-3-3 protein associated with granule membranes was partially removed by a high-salt/carbonate wash, and the membranes could bind further gamma from cytosol or from a purified brain 14-3-3 protein mixture. The binding of gamma 14-3-3 was not Ca(2+)-dependent, nor was it affected by phorbol ester, GTP analogues or cyclic AMP. Using pure phospholipid vesicles it was found that gamma and also epsilon 14-3-3 proteins bound directly to phospholipids. Little binding of brain beta, eta or zeta to phospholipid vesicles was detected. Brain 14-3-3 proteins were also able to aggregate phospholipid vesicles. Recombinant 14-3-3 isoforms (tau and the Xenopus protein) were able to stimulate Ca(2+)-dependent exocytosis in digitonin-permeabilized chromaffin cells. The Xenopus proteins lacks part of the extreme N-terminus, indicating that this domain is not essential for function in exocytosis.
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