ArticleThe Biochemical journal1994
Identification of the vinculin-binding site in the cytoskeletal protein alpha-actinin.
Article in The Biochemical journal, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 82 citations in OpenAlex.
- Structural and functional insights into α-actinin isoforms and their implications in cardiovascular disease.The Journal of general physiology · 2025Review
- Direct investigation of cell contraction signal networks by light-based perturbation methods.Pflugers Archiv : European journal of physiology · 2023Review
- A Layered View on Focal Adhesions.Biology · 2021Review
- Growth factor dependent changes in nanoscale architecture of focal adhesions.Scientific reports · 2021Article
- Dynamics and distribution of paxillin, vinculin, zyxin and VASP depend on focal adhesion location and orientation.Scientific reports · 2019Article
- ACTN2 mutations cause "Multiple structured Core Disease" (MsCD).Acta neuropathologica · 2019Article
- Myosin-1c promotes E-cadherin tension and force-dependent recruitment of α-actinin to the epithelial cell junction.Journal of cell science · 2018Article
- Increased α-actinin-1 destabilizes E-cadherin-based adhesions and associates with poor prognosis in basal-like breast cancer.PloS one · 2018Article
- Live-Cell Super-resolution Reveals F-Actin and Plasma Membrane Dynamics at the T Cell Synapse.Biophysical journal · 2017Article
- Synaptopodin couples epithelial contractility to α-actinin-4-dependent junction maturation.The Journal of cell biology · 2015Article
- Review
- Integrin-dependent force transmission to the extracellular matrix by α-actinin triggers adhesion maturation.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Article
- A molecular trajectory of α-actinin activation.Biophysical journal · 2012Article
- Myosin II activity dependent and independent vinculin recruitment to the sites of E-cadherin-mediated cell-cell adhesion.BMC cell biology · 2011Article
- Dynamics of leading lamellae of living fibroblasts visualized by high-speed scanning probe microscopy.Histochemistry and cell biology · 2010Article
- The talin rod IBS2 alpha-helix interacts with the beta3 integrin cytoplasmic tail membrane-proximal helix by establishing charge complementary salt bridges.The Journal of biological chemistry · 2008Article
- Phosphoinositide binding to the substrate regulates susceptibility to proteolysis by calpain.The Journal of biological chemistry · 2008Article
- Vinculin binding in its closed conformation by a helix addition mechanism.The EMBO journal · 2007Article
- Structural mimicry for vinculin activation by IpaA, a virulence factor of Shigella flexneri.EMBO reports · 2006Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Using low-speed sedimentation equilibrium we have established that vinculin binds to alpha-actinin with a Kd of 1.3 x 10(-5) M. Electron microscopy of negatively stained preparations of vinculin revealed spherical particles (diameter 11.2 nm; S.D. 1.7 nm, n = 21), whereas alpha-actinin appeared as a rod-shaped particle (length 33 nm; S.D. 3.3 nm, n = 23). Mixtures of the two proteins contained both 'lollipop'- and 'dumbell'-shaped particles which we interpret as either one or two spherical vinculin molecules associated with the ends of the alpha-actinin rod. We have further defined the vinculin-binding site in alpha-actinin using 125I-vinculin and a gel-blot assay in which proteolytic fragments of alpha-actinin and fragments of alpha-actinin expressed in Escherichia coli were resolved by SDS/PAGE and blotted to nitrocellulose. 125I-vinculin bound to polypeptides derived from the spectrin-like repeat region of alpha-actinin, but did not bind to the actin-binding domain. Binding was inhibited by a 100-fold molar excess of unlabelled vinculin. Using a series of glutathione S-transferase fusion proteins we have mapped the vinculin-binding site to a region toward the C-terminal end of the molecule (alpha-actinin residues 713-749). 125I-vinculin also bound to fusion proteins containing this sequence which had been immobilized on glutathione-agarose beads. The vinculin-binding site is localized in a highly conserved region of the molecule close to the first of two EF-hand calcium-binding motifs.
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