ArticleParasitology research1994
The use of a water-soluble formazan complex to quantitate the cell number and mitochondrial function of Leishmania major promastigotes.
Article in Parasitology research, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
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- Anti-inflammatory and Cytotoxic Triterpenes from the Rot Roots of Panax notoginseng.Natural products and bioprospecting · 2019Article
- Effect of strontium substitution on the cytocompatibility and 3-D scaffold structure for the xSrO-(10-x) MgO-60SiOJournal of materials science. Materials in medicine · 2017Article
- Pseudotannins self-assembled into antioxidant complexes.Soft matter · 2015Article
- In vitro antileishmanial activity of methanolic and aqueous extracts of Eucalyptus camaldulensis against Leishmania major.Journal of parasitic diseases : official organ of the Indian Society for Parasitology · 2015Article
- Receptor activator for nuclear factor-κB ligand signaling promotes progesterone-mediated estrogen-induced mammary carcinogenesis.Cancer science · 2015Article
- Comparison of tetrazolium salt assays for evaluation of drug activity against Leishmania spp.Journal of clinical microbiology · 2014Article
- Simple colorimetric trypanothione reductase-based assay for high-throughput screening of drugs against Leishmania intracellular amastigotes.Antimicrobial agents and chemotherapy · 2014Article
- Organometallic Pyridylnaphthalimide Complexes as Protein Kinase Inhibitors.Organometallics · 2011Article
- Use of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide for rapid detection of rifampin-resistant Mycobacterium tuberculosis.Journal of clinical microbiology · 1998Article
- Use of Toxoplasma gondii expressing beta-galactosidase for colorimetric assessment of drug activity in vitro.Antimicrobial agents and chemotherapy · 1997Article
- An axenic amastigote system for drug screening.Antimicrobial agents and chemotherapy · 1997Article
- The antileishmanial agent licochalcone A interferes with the function of parasite mitochondria.Antimicrobial agents and chemotherapy · 1995Article
- Article
- Improved attachment and spreading in primary cell cultures of the eastern oyster, Crassostrea virginica.In vitro cellular & developmental biology. AnimalArticle
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5 authors.
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No grant is acknowledged in the PubMed record.
Abstract
One of the methods to quantitate Leishmania major promastigotes (LmP) has been to utilize the formation of a formazan dye, which in turn is produced via conversion of an artificial substrate, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). The method has one major drawback in that the formazan complex precipitates inside the parasites and has to be extracted by denaturants before measurements can be performed. By using a new synthetic substrate, 3-(4,5-dimethylthiazol-2-yl)-5- (3-carboxymethoxyphenyl)-2-(4-sulfonyl)-2H-tetrazolium (MTS), the extraction procedure is eliminated as the formazan-like dye is released spontaneously into the medium, making it possible to perform several measurements on the same parasite culture without disturbing or killing the parasites. The measurements were shown to reflect the numbers of parasites as confirmed via comparative experiments using radioactive thymidine uptake and cell counting, respectively. The method is simple, fast, and highly reproducible and is suitable for drug screening, identification of drug-resistant isolates, and growth-kinetics studies. It is therefore contemplated that the MTS method will be a general and useful technique in this field of parasitology.
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