ArticleNucleic acids research1994
DNA damage by anti-cancer agents resolved at the nucleotide level of a single copy gene: evidence for a novel binding site for cisplatin in cells.
Article in Nucleic acids research, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed, 45 citations in OpenAlex.
- Methodologies for detecting environmentally induced DNA damage and repair.Environmental and molecular mutagenesis · 2020Review
- Predicting Drug Combination Index and Simulating the Network-Regulation Dynamics by Mathematical Modeling of Drug-Targeted EGFR-ERK Signaling Pathway.Scientific reports · 2017Article
- Base-Resolution Analysis of Cisplatin-DNA Adducts at the Genome Scale.Angewandte Chemie (International ed. in English) · 2016Article
- Characterisation of the DNA sequence specificity, cellular toxicity and cross-linking properties of novel bispyridine-based dinuclear platinum complexes.BMC cancer · 2016Article
- Mechanisms of drug combinations: interaction and network perspectives.Nature reviews. Drug discovery · 2009Review
- Nucleotide-resolution mapping of topoisomerase-mediated and apoptotic DNA strand scissions at or near an MLL translocation hotspot.American journal of human genetics · 2006Article
- PCR-based methods for detecting DNA damage and its repair at the sub-gene and single nucleotide levels in cells.Molecular biotechnology · 2002Article
- The effect of chromatin structure on cisplatin damage in intact human cells.Nucleic acids research · 2000Article
- The interaction of DNA-targeted platinum phenanthridinium complexes with DNA.Nucleic acids research · 1998Article
- Identifying 5-methylcytosine and related modifications in DNA genomes.Nucleic acids research · 1998Review
- Superhelical torsion controls DNA interstrand cross-linking by antitumor cis- diamminedichloroplatinum(II).Nucleic acids research · 1996Article
- Strand-specific measurement of cisplatin-induced DNA damage and repair using quantitative PCR.Nucleic acids research · 1996Article
- Detection of platinum-DNA adducts by 32P-postlabelling.Nucleic acids research · 1995Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A new PCR based technique has been developed to investigate the sequence selectivity of adduct formation by DNA damaging agents in a single copy gene in isolated genomic DNA or in drug treated cells. Single-strand ligation PCR (sslig-PCR) demonstrated that cisplatin and nitrogen mustards reacted with guanine in an N-ras fragment with varying sequence specificity similar to that observed previously in plasmid DNA. In cisplatin-treated cells sslig-PCR demonstrated all the adducts found in isolated DNA and with the same sequence selectivity showing a preference for GG and AG sites. However, in cells an additional site of DNA binding of cisplatin was observed at the two occurrences of the sequence 5'-TACT-3' on the transcribed and non-transcribed strands. This sequence is not a recognised target for cisplatin and represents a novel adduct formed in cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.