ArticleMolecular and cellular biology1994
Sequences controlling transcription of the Chlamydomonas reinhardtii beta 2-tubulin gene after deflagellation and during the cell cycle.
Article in Molecular and cellular biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 72 citations in OpenAlex.
- A tubulin-MAPKKK pathway engages tubulin isotype interaction for neuroprotection.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Cilia and transcription: a mini review.Frontiers in cell and developmental biology · 2025Review
- Review
- Role of intraflagellar transport in transcriptional control during flagellar regeneration inMolecular biology of the cell · 2023Article
- Regulation of Tubulin Gene Expression: From Isotype Identity to Functional Specialization.Frontiers in cell and developmental biology · 2022Review
- CrMAPK3 regulates the expression of iron-deficiency-responsive genes in Chlamydomonas reinhardtii.BMC biochemistry · 2017Article
- Identification and Characterization of a cis-Regulatory Element for Zygotic Gene Expression in Chlamydomonas reinhardtii.G3 (Bethesda, Md.) · 2016Article
- Prevalence, evolution, and cis-regulation of diel transcription in Chlamydomonas reinhardtii.G3 (Bethesda, Md.) · 2014Article
- A C2H2 zinc finger protein FEMU2 is required for fox1 expression in Chlamydomonas reinhardtii.PloS one · 2014Article
- Article
- Fe sparing and Fe recycling contribute to increased superoxide dismutase capacity in iron-starved Chlamydomonas reinhardtii.The Plant cell · 2012Article
- Regulation of the expression of H43/Fea1 by multi-signals.Photosynthesis research · 2011Article
- Expression of a low CO₂-inducible protein, LCI1, increases inorganic carbon uptake in the green alga Chlamydomonas reinhardtii.The Plant cell · 2010Article
- Two Chlamydomonas CTR copper transporters with a novel cys-met motif are localized to the plasma membrane and function in copper assimilation.The Plant cell · 2009Article
- Mutations in radial spoke head protein genes RSPH9 and RSPH4A cause primary ciliary dyskinesia with central-microtubular-pair abnormalities.American journal of human genetics · 2009Article
- Two iron-responsive promoter elements control expression of FOX1 in Chlamydomonas reinhardtii.Eukaryotic cell · 2007Article
- FEA1, FEA2, and FRE1, encoding two homologous secreted proteins and a candidate ferrireductase, are expressed coordinately with FOX1 and FTR1 in iron-deficient Chlamydomonas reinhardtii.Eukaryotic cell · 2007Article
- Flagellar elongation and gene expression in Chlamydomonas reinhardtii.Eukaryotic cell · 2007Article
- REP27, a tetratricopeptide repeat nuclear-encoded and chloroplast-localized protein, functions in D1/32-kD reaction center protein turnover and photosystem II repair from photodamage.Plant physiology · 2007Article
- Identification of a plastid response element that acts as an enhancer within the Chlamydomonas HSP70A promoter.Nucleic acids research · 2006Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In Chlamydomonas reinhardtii, transcripts from the beta 2-tubulin gene (tubB2), as well as those from other tubulin-encoding genes, accumulate immediately after flagellar excision as well as at a specific time in the cell cycle. Control of tubB2 transcript accumulation following deflagellation is regulated, at least partially, at the transcriptional level. We have fused the tubB2 promoter to the arylsulfatase (ars) reporter gene, introduced this construct into C. reinhardtii, and compared expression of the chimeric gene with that of the endogenous tubB2 gene. After flagellar excision, transcripts from the tubB2/ars chimeric gene accumulate with kinetics similar to those of transcripts from the endogenous tubB2 gene. The tubB2/ars transcripts also accumulate in a cell cycle-specific manner; however, chimeric transcripts are more abundant earlier in the cell cycle than the endogenous tubB2 transcripts. To elucidate transcriptional control of tubB2, we have mutated or removed sequences in the tubB2 promoter and examined the effect on transcription. The tubB2 promoter shares features with the promoters of other tubulin-encoding genes; these include a GC-rich region between the TATA box and the transcription initiation site and multiple copies of a 10-bp sequence motif that we call the tub box. The tubB2 gene contains seven tub box motifs. Changing the GC-rich region to an AT-rich region or removing three of the seven tub box motifs did not significantly affect transcription of the chimeric gene. However, removing four or five tub box motifs prevented increased transcription following deflagellation and diminished cell cycle-regulated transcription from the tubB2 promoter.
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