ArticleMolecular and cellular biology1994
Differential roles of heat shock protein 70 in the in vitro nuclear import of glucocorticoid receptor and simian virus 40 large tumor antigen.
Article in Molecular and cellular biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 114 citations in OpenAlex.
- Choosing the right partner in hormone-dependent gene regulation: Glucocorticoid and progesterone receptors crosstalk in breast cancer cells.Frontiers in endocrinology · 2022Review
- Defective heat shock factor 1 inhibits the growth of fibrosarcoma derived from simian virus 40/T antigen‑transformed MEF cells.Molecular medicine reports · 2015Article
- 70-kDa heat shock cognate protein hsc70 mediates calmodulin-dependent nuclear import of the sex-determining factor SRY.The Journal of biological chemistry · 2013Article
- Neuronal hypoxia induces Hsp40-mediated nuclear import of type 3 deiodinase as an adaptive mechanism to reduce cellular metabolism.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- HSP70 inhibition by the small-molecule 2-phenylethynesulfonamide impairs protein clearance pathways in tumor cells.Molecular cancer research : MCR · 2011Article
- RanGTPase: A Key Regulator of Nucleocytoplasmic Trafficking.Molecular and cellular pharmacology · 2009Article
- Proteasomal inhibition enhances glucocorticoid receptor transactivation and alters its subnuclear trafficking.Molecular and cellular biology · 2002Article
- FANCC interacts with Hsp70 to protect hematopoietic cells from IFN-gamma/TNF-alpha-mediated cytotoxicity.The EMBO journal · 2001Article
- Heat shock protein 90 and the nuclear transport of progesterone receptor.Cell stress & chaperones · 2001Article
- Role of chaperones in nuclear translocation and transactivation of steroid receptors.Endocrine · 2001Review
- Chaperones in cell cycle regulation and mitogenic signal transduction: a review.Cell proliferation · 2000Review
- Subcellular compartmentalization of E2F family members is required for maintenance of the postmitotic state in terminally differentiated muscle.The Journal of cell biology · 2000Article
- p53-independent association between SV40 large T antigen and the major cytosolic heat shock protein, HSP90.Oncogene · 2000Article
- Quantitative assessment of complex formation of nuclear-receptor accessory proteins.The Biochemical journal · 2000Article
- The DnaJ domain of polyomavirus large T antigen is required to regulate Rb family tumor suppressor function.Journal of virology · 1997Article
- Tiny T antigen: an autonomous polyomavirus T antigen amino-terminal domain.Journal of virology · 1997Article
- Subnuclear trafficking of glucocorticoid receptors in vitro: chromatin recycling and nuclear export.The Journal of cell biology · 1997Article
- A role for HDJ-2/HSDJ in correcting subnuclear trafficking, transactivation, and transrepression defects of a glucocorticoid receptor zinc finger mutant.Molecular biology of the cell · 1997Article
- The T/t common exon of simian virus 40, JC, and BK polyomavirus T antigens can functionally replace the J-domain of the Escherichia coli DnaJ molecular chaperone.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Induction of HSP70 and polyubiquitin expression associated with plant virus replication.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
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2 authors at 1 institution in 1 country.
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Abstract
Nuclear import of glucocorticoid receptors (GRs) was analyzed in vitro with digitonin-permeabilized cells (S. A. Adam, R. Sterne-Marr, and L. Gerace, J. Cell Biol. 111:807-816, 1990). Indirect immunofluorescence methods were used to monitor the transport of GRs from rat hepatoma and fibroblast cell cytosol into HeLa nuclei. In vitro nuclear import of GRs was shown to be hormone dependent and to require ATP and incubation at ambient temperatures (i.e., 30 degrees C). Hormone-dependent dissociation of GR-bound proteins, such as the 90-kDa heat shock protein, hsp90, is part of an activation process that is obligatory for the expression of the receptor's DNA-binding activity. Inhibition of in vitro GR activation by Na2MoO4 blocked hormone-dependent nuclear import, demonstrating that receptor activation is required for nuclear import. The addition to GR-containing cytosol of antiserum directed against the cytosolic 70-kDa heat shock protein, hsp70, while effective in blocking the nuclear import of simian virus 40 large tumor antigen (SV40 TAg), did not affect hormone-dependent nuclear import of endogenous, full-length GRs or an exogenously added truncated GR protein (i.e., XGR556) that lacks a hormone-binding domain but possesses a constitutively active nuclear localization signal sequence (NLS). Depletion of hsp70 from HeLa cell cytosol did not affect the nuclear import of exogenously added XGR556 but led to inhibition of SV40 TAg nuclear import. Thus, two closely related NLSs, one contained within GRs and the other contained within SV40 TAg, are distinguished by their differential requirements for hsp70 in vitro.
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