ArticleThe Journal of cell biology1994
Mapping actin surfaces required for functional interactions in vivo.
Article in The Journal of cell biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
35 citing papers in PubMed, 62 citations in OpenAlex.
- The actin cytoskeletal network plays a role in yeast prion transmission and contributes to prion stability.Molecular microbiology · 2020Article
- Genetic suppression of defective profilin by attenuated Myosin II reveals a potential role for Myosin II in actin dynamics in vivo in fission yeast.Molecular biology of the cell · 2020Article
- Article
- Fimbrin phosphorylation by metaphase Cdk1 regulates actin cable dynamics in budding yeast.Nature communications · 2016Article
- Interactions between the yeast SM22 homologue Scp1 and actin demonstrate the importance of actin bundling in endocytosis.The Journal of biological chemistry · 2008Article
- The yeast actin cytoskeleton: from cellular function to biochemical mechanism.Microbiology and molecular biology reviews : MMBR · 2006Review
- The BAR domain proteins: molding membranes in fission, fusion, and phagy.Microbiology and molecular biology reviews : MMBR · 2006Review
- Alanine scanning of Arp1 delineates a putative binding site for Jnm1/dynamitin and Nip100/p150Glued.Molecular biology of the cell · 2005Article
- Remodeling of organelle-bound actin is required for yeast vacuole fusion.The Journal of cell biology · 2002Article
- Stu1p is physically associated with beta-tubulin and is required for structural integrity of the mitotic spindle.Molecular biology of the cell · 2002Article
- An atomic model of actin filaments cross-linked by fimbrin and its implications for bundle assembly and function.The Journal of cell biology · 2001Article
- Candida albicans INT1-induced filamentation in Saccharomyces cerevisiae depends on Sla2p.Molecular and cellular biology · 2001Article
- Generation of an isogenic collection of yeast actin mutants and identification of three interrelated phenotypes.Genetics · 2001Article
- Pan1p, End3p, and S1a1p, three yeast proteins required for normal cortical actin cytoskeleton organization, associate with each other and play essential roles in cell wall morphogenesis.Molecular and cellular biology · 2000Article
- Aip1p interacts with cofilin to disassemble actin filaments.The Journal of cell biology · 1999Article
- Analysis of mutations in the yeast mRNA decapping enzyme.Genetics · 1999Article
- GCS1, an Arf guanosine triphosphatase-activating protein in Saccharomyces cerevisiae, is required for normal actin cytoskeletal organization in vivo and stimulates actin polymerization in vitro.Molecular biology of the cell · 1999Article
- The multiple roles of Cyk1p in the assembly and function of the actomyosin ring in budding yeast.Molecular biology of the cell · 1999Article
- Regulation of the actin cytoskeleton organization in yeast by a novel serine/threonine kinase Prk1p.The Journal of cell biology · 1999Article
- Alf1p, a CLIP-170 domain-containing protein, is functionally and physically associated with alpha-tubulin.The Journal of cell biology · 1999Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
An in vivo strategy to identify amino acids of actin required for functional interactions with actin-binding proteins was developed. This approach is based on the assumption that an actin mutation that specifically impairs the interaction with an actin-binding protein will cause a phenotype similar to a null mutation in the gene that encodes the actin-binding protein. 21 actin mutations were analyzed in budding yeast, and specific regions of actin subdomain 1 were implicated in the interaction with fimbrin, an actin filament-bundling protein. Mutations in this actin subdomain were shown to be, like a null allele of the yeast fimbrin gene (SAC6), lethal in combination with null mutations in the ABP1 and SLA2 genes, and viable in combination with a null mutation in the SLA1 gene. Biochemical experiments with act1-120 actin (E99A, E100A) verified a defect in the fimbrin-actin interaction. Genetic interactions between mutant alleles of the yeast actin gene and null alleles of the SAC6, ABP1, SLA1, and SLA2 genes also demonstrated that the effects of the 21 actin mutations are diverse and allowed four out of seven pseudo-wild-type actin alleles to be distinguished from the wild-type gene for the first time, providing evidence for functional redundancy between different surfaces of actin.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.