ArticleArchives of virology1994
Pestiviruses isolated from pigs, cattle and sheep can be allocated into at least three genogroups using polymerase chain reaction and restriction endonuclease analysis.
Article in Archives of virology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 185 papers.
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Who cites it
185 citing papers in PubMed.
- Molecular diversity and detection of pestiviruses across ruminant species in Türkiye.Tropical animal health and production · 2026Article
- Isolation and partial 5'UTR-based molecular characterization of bovine enterovirus F from non-diarrheic cattle in Türkiye.Molecular biology reports · 2026Article
- An outbreak of septicemic listeriosis associated with diarrhea in lactating dairy cows.The Journal of veterinary medical science · 2026Article
- Ag-rich Ag-Au nanoshells based lateral flow immunoassay for rapid and sensitive detection of bovine viral diarrhea virus.Mikrochimica acta · 2026Article
- Molecular detection and genetic characterization of pestiviruses associated with abortion in domestic small ruminants in Türkiye.Virus genes · 2026Article
- Genomic Surveillance of BVDV in Southern Brazil: What Changed After a Decade in Rio Grande do Sul?Viruses · 2026Article
- First isolation report of bovine adenovirus type 10 in Japan: virological and pathological characteristics.The Journal of veterinary medical science · 2026Article
- Isolation and identification of a potential new bovine viral diarrhea virus genotype associated with severe outbreaks in yaks (Frontiers in microbiology · 2026Article
- Outbreak of BVDV-1b associated with persistent infection, reproductive losses, and congenital malformations in beef cattle in Southern Brazil.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2025Article
- Detection of Bovine Viral Diarrhoea Virus in a Case Series of Clinically Cachectic Cattle from Tiaret, Algeria.Veterinary sciences · 2025Article
- Evaluation of Risk Determinants and Molecular Characterisation for Non-Primate Hepacivirus Infection in Turkish Horses.Pathogens (Basel, Switzerland) · 2025Article
- Droplet digital PCR (ddPCR) for enhanced detection and quantification of bovine viral diarrhea virus (BVDV): A promising tool for disease control.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2025Article
- Fecal Sample Surveillance of the Wildlife Virome in Central Italy: Insights from the Foreste Casentinesi National Park.Animals : an open access journal from MDPI · 2025Article
- Epidemiology and Evolution of Bovine Viral Diarrhea Virus (BVDV) in Uruguay: A 10-Year Study.Viruses · 2025Article
- Improved method for elimination of bovine viral diarrhea virus from contaminated cell lines using virus inhibitor DB772.The Journal of veterinary medical science · 2025Article
- Article
- Detection of bovine respiratory disease complex-related pathogens in nasopharynx-associated lymphoid tissue.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2025Article
- Article
- Genetic Variability of Pestivirus A (BVDV-1) Circulating in Cattle From Eastern Turkey.Veterinary medicine and science · 2025Article
- Ear tissue as a diagnostic sample for pestivirus detection in semi-domesticated Eurasian tundra reindeer (Frontiers in microbiology · 2025Article
125 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A polymerase chain reaction-based assay capable of detecting a broad range of pestiviruses from pigs, cattle, or sheep was developed. Of six sets of primers selected from different parts of the pestivirus genome, the best results were provided by a pair from the highly conserved 5' non-coding region which gave amplification with all 129 isolates tested. This panel consisted of 33 isolates from pigs, 79 from cattle, and 17 from sheep. Differentiation between the viruses was achieved by cutting the PCR-amplified products with the restriction endonucleases AvaI and Bg1I. Using this procedure it was possible to distinguish at least 3 genogroups; group 1 (HCV) contained 32 of the pig isolates, group II (BVDV) contained all the cattle isolates tested plus 6 sheep isolates and group III (BDV) contained 11 sheep isolates and 1 pig isolate.
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