ArticlePlant physiology1994
Analysis of the involvement of ocs-like bZip-binding elements in the differential strength of the bidirectional mas1'2' promoter.
Article in Plant physiology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed.
- The regulation ofPlant direct · 2019Article
- Epitope-tagged protein-based artificial miRNA screens for optimized gene silencing in plants.Nature protocols · 2014Article
- Analysis of the spacing between the two palindromes of activation sequence-1 with respect to binding to different TGA factors and transcriptional activation potential.Nucleic acids research · 2002Article
- A 42 bp fragment of the pmas1' promoter containing an ocs-like element confers a developmental, wound- and chemically inducible expression pattern.Plant molecular biology · 1998Article
- Cooperative DNA binding and sequence discrimination by the Opaque2 bZIP factor.The Plant cell · 1998Article
- An as-1-like motif controls the level of expression of the gene for the pathogenesis-related protein 1a from tobacco.Plant molecular biology · 1998Article
- The maize gene liguleless2 encodes a basic leucine zipper protein involved in the establishment of the leaf blade-sheath boundary.Genes & development · 1998Article
- The molecular and functional characterization of an Opaque2 homologue gene from Coix and a new classification of plant bZIP proteins.Plant molecular biology · 1998Article
- Binding site requirements and differential representation of TGF factors in nuclear ASF-1 activity.Nucleic acids research · 1995Article
- A dominant negative mutant of PG13 suppresses transcription from a cauliflower mosaic virus 35S truncated promoter in transgenic tobacco plants.The Plant cell · 1994Article
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4 authors.
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Abstract
The ocs-like elements of the bidirectional mas1'2' promoter of Agrobacterium tumefaciens, mas1' and mas2', were analyzed to elucidate their role in the expression conferred by this promoter. Tetramers of the elements were cloned upstream of the beta-glucuronidase-coding region linked to the 35S promoter deleted at -54. Transient expression assays with tobacco (Nicotiana tabacum) and potato (Solanum tuberosum) protoplasts showed that tetramers of the mas1' element had 3- to 8-fold enhancing activity, respectively. Enhancement obtained by tetramers of the mas2' element was higher, suggesting that this element plays a role in the stronger promoter activity from the 2' side. Three cDNA clones with higher homology to the tobacco transcription factor TGA1a were isolated from a potato root expression library. Overexpression of the proteins encoded by these cDNA clones in Escherichia coli and analysis of DNA-binding activity in bacterial extracts showed that all three factors could bind strongly to the mas1' ocs-like element. In contrast, only two of the mas-binding factors exhibited significant binding to the mas2' element. Southern analysis revealed the presence of a small, multigene family encoding the mas-binding factors in potato.
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