ArticlePlant physiology1993
Generation of expressed sequence tags of random root cDNA clones of Brassica napus by single-run partial sequencing.
Article in Plant physiology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Computational analysis of common bean (Phaseolus vulgaris L., genotype BAT93) lycopene β-cyclase and β-carotene hydroxylase gene's cDNA.Bioinformation · 2013Article
- Analysis of beta-carotene hydroxylase gene cDNA isolated from the American oil-palm (Elaeis oleifera) mesocarp tissue cDNA library.Bioinformation · 2010Article
- Comparative analysis of 5,211 leaf ESTs of wild rice (Oryza minuta).Plant cell reports · 2004Article
- Article
- Dominant negative guard cell K+ channel mutants reduce inward-rectifying K+ currents and light-induced stomatal opening in arabidopsis.Plant physiology · 2001Article
- REP1, a basic helix-loop-helix protein, is required for a branch pathway of phytochrome A signaling in arabidopsis.The Plant cell · 2000Article
- Expression analysis of ESts derived from the inner bark of Cryptomeria japonica.Plant molecular biology · 2000Article
- Toward a functional catalog of the plant genome. A survey of genes for lipid biosynthesis.Plant physiology · 2000Article
- Differential expression of senescence-associated mRNAs during leaf senescence induced by different senescence-inducing factors in Arabidopsis.Plant molecular biology · 1998Article
- AtKUP1: an Arabidopsis gene encoding high-affinity potassium transport activity.The Plant cell · 1998Article
- Article
- Expressed sequence tags of Chinese cabbage flower bud cDNA.Plant physiology · 1996Article
- Rapid and transient induction of calmodulin-encoding gene(s) of Brassica napus by a touch stimulus.Plant cell reports · 1996Article
- Characterization of a cDNA encoding cysteine proteinase inhibitor from Chinese cabbage (Brassica campestris L. ssp. pekinensis) flower buds.Plant molecular biology · 1996Article
- Analysis of randomly isolated cDNAs from developing endosperm of rice (Oryza sativa L.): evaluation of expressed sequence tags, and expression levels of mRNAs.Plant molecular biology · 1995Article
- Expressed Sequence Tags from Developing Castor Seeds.Plant physiology · 1995Article
- Frequent in-frame length variations are found in the diverged simple repeat sequences of the protein-coding regions of two putative protein kinase genes of Brassica napus.Plant molecular biology · 1995Article
- Functional complementation of a yeast vesicular transport mutation ypt1-1 by a Brassica napus cDNA clone encoding a small GTP-binding protein.Plant molecular biology · 1994Article
- Partial sequencing and mapping of clones from two maize cDNA libraries.Plant molecular biology · 1994Article
- Expressed sequence tags from cultured cells of rice (Oryza sativa L.) under stressed conditions: analysis of transcripts of genes engaged in ATP-generating pathways.Plant molecular biology · 1994Article
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8 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Two hundred thirty-seven expressed sequence tags (ESTs) of Brassica napus were generated by single-run partial sequencing of 197 random root cDNA clones. A computer search of these root ESTs revealed that 21 ESTs show significant similarity to the protein-coding sequences in the existing data bases, including five stress- or defense-related genes and four clones related to the genes from other kingdoms. Northern blot analysis of the 10 data base-matched cDNA clones revealed that many of the clones are expressed most abundantly in root but less abundantly in other organs. However, two clones were highly root specific. The results show that generation of the root ESTs by partial sequencing of random cDNA clones along with the expression analysis is an efficient approach to isolate genes that are functional in plant root in a large scale. We also discuss the results of the examination of cDNA libraries and sequencing methods suitable for this approach.
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