ArticleNucleic acids research1994
Interaction between the first and last nucleotides of pre-mRNA introns is a determinant of 3' splice site selection in S. cerevisiae.
Article in Nucleic acids research, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 44 citations in OpenAlex.
- Splicing factor Prp18p promotes genome-wide fidelity of consensus 3'-splice sites.Nucleic acids research · 2023Article
- U5 snRNA Interactions With Exons Ensure Splicing Precision.Frontiers in genetics · 2021Article
- Structures of the human spliceosomes before and after release of the ligated exon.Cell research · 2019Article
- Functional roles of protein splicing factors.Bioscience reports · 2012Review
- Nonsense-mediated mRNA decay mutes the splicing defects of spliceosome component mutations.RNA (New York, N.Y.) · 2009Article
- Widespread impact of nonsense-mediated mRNA decay on the yeast intronome.Molecular cell · 2008Article
- The Prp18 protein stabilizes the interaction of both exons with the U5 snRNA during the second step of pre-mRNA splicing.Genes & development · 2007Article
- Comprehensive splice-site analysis using comparative genomics.Nucleic acids research · 2006Article
- A mutational analysis of U12-dependent splice site dinucleotides.RNA (New York, N.Y.) · 2005Article
- Genetic interactions between the 5' and 3' splice site consensus sequences and U6 snRNA during the second catalytic step of pre-mRNA splicing.RNA (New York, N.Y.) · 2001Article
- Evidence for a role of Sky1p-mediated phosphorylation in 3' splice site recognition involving both Prp8 and Prp17/Slu4.RNA (New York, N.Y.) · 2001Article
- Role of the 3' splice site in U12-dependent intron splicing.Molecular and cellular biology · 2001Article
- Role of adenine functional groups in the recognition of the 3'-splice-site AG during the second step of pre-mRNA splicing.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Allele-specific genetic interactions between Prp8 and RNA active site residues suggest a function for Prp8 at the catalytic core of the spliceosome.Genes & development · 1999Article
- AT-AC pre-mRNA splicing mechanisms and conservation of minor introns in voltage-gated ion channel genes.Molecular and cellular biology · 1999Review
- Detection of a novel ATP-dependent cross-linked protein at the 5' splice site-U1 small nuclear RNA duplex by methylene blue-mediated photo-cross-linking.Molecular and cellular biology · 1998Article
- Use of alternate splice sites in granule-bound starch synthase mRNA from low-amylose rice varieties.Plant molecular biology · 1998Article
- A role for U2/U6 helix Ib in 5' splice site selection.RNA (New York, N.Y.) · 1998Article
- Genetic interaction between U6 snRNA and the first intron nucleotide in Saccharomyces cerevisiae.RNA (New York, N.Y.) · 1998Article
- The role of branchpoint-3' splice site spacing and interaction between intron terminal nucleotides in 3' splice site selection in Saccharomyces cerevisiae.The EMBO journal · 1997Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The splicing of group II and nuclear pre-mRNAs introns occurs via a similar splicing pathway and some of the RNA-RNA interactions involved in these splicing reactions show structural similarities. Recently, genetic analyses performed in a group II intron and the yeast nuclear actin gene suggested that non Watson-Crick interactions between intron boundaries are important for the second splicing step efficiency in both classes of introns. We here show that, in the yeast nuclear rp51A intron, a G to A mutation at the first position activates cryptic 3' splice sites with the sequences UAC/ or UAA/. Moreover, the natural 3' splice site could be reactivated by a G to C substitution of the last intron nucleotide. These results demonstrate that the interaction between the first and last intron nucleotides is a conserved feature of nuclear pre-mRNA splicing in yeast and is involved in the mechanism of 3' splice site selection.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.