ArticleNucleic acids research1994
Functional analysis of cis-acting DNA elements required for expression of the SL RNA gene in the parasitic protozoan Leishmania amazonensis.
Article in Nucleic acids research, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed, 42 citations in OpenAlex.
- Article
- Regulation of gene expression in protozoa parasites.Journal of biomedicine & biotechnology · 2010Review
- A novel class of developmentally regulated noncoding RNAs in Leishmania.Eukaryotic cell · 2006Article
- Trypanosomal TBP functions with the multisubunit transcription factor tSNAP to direct spliced-leader RNA gene expression.Molecular and cellular biology · 2005Article
- trans and cis splicing in trypanosomatids: mechanism, factors, and regulation.Eukaryotic cell · 2003Review
- RNA polymerase II-dependent transcription in trypanosomes is associated with a SNAP complex-like transcription factor.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Unconventional rules of small nuclear RNA transcription and cap modification in trypanosomatids.Gene expression · 2002Review
- Trypanosome spliced leader RNA genes contain the first identified RNA polymerase II gene promoter in these organisms.Nucleic acids research · 2001Article
- Transcription termination and 3'-End processing of the spliced leader RNA in kinetoplastids.Molecular and cellular biology · 1999Article
- Structure-function analysis of the trypanosomatid spliced leader RNA.Nucleic acids research · 1998Article
- Characterization of a novel trypanosomatid small nucleolar RNA.Nucleic acids research · 1998Article
- Spliced leader RNA of trypanosomes: in vivo mutational analysis reveals extensive and distinct requirements for trans splicing and cap4 formation.The EMBO journal · 1996Article
- Control of gene expression in trypanosomes.Microbiological reviews · 1995Review
- A conserved upstream element is essential for transcription of the Leishmania tarentolae mini-exon gene.The EMBO journal · 1994Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA sequences, that control expression of the spliced leader (SL) RNA gene in the parasitic protozoan Leishmania amazonensis, were mapped by block substitution mutagenesis. In the absence of a functional in vitro system for transcription, no promoter elements have yet been identified in this organism. We therefore developed an alternative in vivo approach, in which the SL RNA gene was tagged and then subjected to a series of linker scanning mutations. Each tagged and mutated SL RNA construct was introduced into parasite cells via the pX transfection vector, and was examined for expression of the tagged SL RNA followed by characterization of its transcriptional start site. The replacement of a critical DNA element was expected to prevent expression of the tagged SL RNA. We found that the putative SL RNA promoter is complex and includes two elements: one is located upstream to the coding region, between positions -30 to -70; and the other is located between -10 to +10, and includes transcribed sequences. In addition to the functional relationship between the SL RNA and vertebrate U snRNAs, we found structural similarities in their regulatory elements, which may possibly indicate a common evolutionary ancestry for these molecules.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.