ArticleJournal of clinical pathology1994
Detection of tumour necrosis factor alpha in sarcoidosis and tuberculosis granulomas using in situ hybridisation.
Article in Journal of clinical pathology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed, 44 citations in OpenAlex.
- Maintenance treatment with infliximab for ulcerative ileitis after intestinal transplantation: A case report.World journal of clinical cases · 2021Article
- Anti-TNF Therapy in Crohn's Disease.International journal of molecular sciences · 2018Review
- Infliximab for treatment of granulomatous disease in patients with common variable immunodeficiency.Journal of clinical immunology · 2014Article
- Pleural tuberculosis in patients with early HIV infection is associated with increased TNF-alpha expression and necrosis in granulomas.PloS one · 2009Article
- Analysis of regulatory T cell associated forkhead box P3 expression in the lungs of patients with sarcoidosis.Clinical and experimental immunology · 2008Article
- Cytokine mRNA expression in Mycobacterium ulcerans-infected human skin and correlation with local inflammatory response.Infection and immunity · 2006Article
- Distribution of IFN-gamma, IL-4 and TNF-alpha protein and CD8 T cells producing IL-12p40 mRNA in human lung tuberculous granulomas.Immunology · 2002Article
- Genotyping in the MHC locus: potential for defining predictive markers in sarcoidosis.Respiratory research · 2002Review
- In situ production of gamma interferon, interleukin-4, and tumor necrosis factor alpha mRNA in human lung tuberculous granulomas.Infection and immunity · 2000Article
- Generation of multinucleated giant cells in vitro by culture of human monocytes with Mycobacterium bovis BCG in combination with cytokine-containing supernatants.Infection and immunity · 1999Article
- Review
- Increased inflammatory cytokines and new collagen formation in cutaneous tuberculosis and sarcoidosis.Thorax · 1996Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimsTo determine the site of tumour necrosis factor alpha (TNF alpha) product and mRNA in granulomas.
methodIn situ hybridisation with digoxigenin labelled or biotinylated oligonucleotide probes was used to demonstrate the presence of total mRNA, and then the presence of TNF alpha mRNA in the biopsy specimens of 37 granulomas (31 sarcoidosis, six tuberculosis).
resultsTNF alpha mRNA was detected in epithelioid cells, giant cells, and lymphocytes in the granulomas. Some sarcoidosis specimens did not contain detectable mRNA for TNF, but did contain TNF peptide in the epithelioid or giant cells on immunostaining. This may have been due to stored TNF present in cells in which mRNA for TNF is no longer being produced.
conclusionThe results suggest that giant cells should not be regarded as effete cells, as they contain large amounts of mRNA and seem to be actively producing TNF alpha.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.