ArticleJournal of clinical microbiology1994
Direct detection of Mycobacterium tuberculosis complex in respiratory specimens by a target-amplified test system.
Article in Journal of clinical microbiology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers, 1 of them a synthesis that pooled it.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
53 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Pooled it
- Proficiency testing of clinical microbiology laboratories using modified decontamination procedures for detection of nontuberculous mycobacteria in sputum samples from cystic fibrosis patients. The Nontuberculous Mycobacteria in Cystic Fibrosis Study Group.Journal of clinical microbiology · 1997Trial
- Performance of an automated Q-beta replicase amplification assay for Mycobacterium tuberculosis in a clinical trial.Journal of clinical microbiology · 1997Trial
- Rapid identification of Mycobacterium tuberculosis complex on urine samples by Gen-Probe amplification test.Urological research · 1997Trial
- Diagnostic value of an amplification method (Gen-Probe) compared with that of culture for diagnosis of tuberculosis.Journal of clinical microbiology · 1995Trial
- Pulmonary Tuberculosis Diagnosis: Where We Are?Tuberculosis and respiratory diseases · 2016Review
- Molecular Mycobacteriology and Expansion in Disease Diagnosis.Indian journal of clinical biochemistry : IJCB · 2016Review
- Pilot study of a rapid and minimally instrumented sputum sample preparation method for molecular diagnosis of tuberculosis.Scientific reports · 2016Article
- Laboratory diagnosis of tuberculosis in resource-poor countries: challenges and opportunities.Clinical microbiology reviews · 2011Review
- Comparison of in-house PCR with conventional techniques and Cobas Amplicor M. tuberculosis kit for detection of Mycobacterium tuberculosis.Yonsei medical journal · 2008Article
- Serum ferroxidase albumin ratio as a marken in pulmonary tuberculosis.Indian journal of clinical biochemistry : IJCB · 2007Article
- Use of equivocal zone in interpretation of results of the amplified mycobacterium tuberculosis direct test for diagnosis of tuberculosis.Journal of clinical microbiology · 2003Article
- Evaluation of BacT/Alert 3D liquid culture system for recovery of mycobacteria from clinical specimens using sodium dodecyl (lauryl) sulfate-NaOH decontamination.Journal of clinical microbiology · 2001Article
- Multicenter evaluation of the Abbott LCx Mycobacterium tuberculosis ligase chain reaction assay.Journal of clinical microbiology · 1999Article
- Evaluation of three nucleic acid amplification methods for direct detection of Mycobacterium tuberculosis complex in respiratory specimens.Journal of clinical microbiology · 1999Article
- Evaluation of the Abbott LCx Mycobacterium tuberculosis assay for direct detection of Mycobacterium tuberculosis complex in human samples.Journal of clinical microbiology · 1999Article
- Rapid diagnosis of pulmonary tuberculosis with the LCx Mycobacterium tuberculosis assay and comparison with conventional diagnostic techniques.Journal of clinical microbiology · 1998Article
- Comparison of Roche Cobas Amplicor Mycobacterium tuberculosis assay with in-house PCR and culture for detection of M. tuberculosis.Journal of clinical microbiology · 1998Article
- Clinical evaluation of a commercial ligase-based gene amplification method for detection of Mycobacterium tuberculosis.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 1998Article
- Detection and identification of Mycobacterium tuberculosis directly from sputum sediments by ligase chain reaction.Journal of clinical microbiology · 1998Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A total of 938 respiratory specimens (633 sputa, 249 bronchial and tracheal aspirates, and 56 bronchoalveolar lavages) from 589 patients were tested for direct detection of Mycobacterium tuberculosis complex by the Gen-Probe amplified Mycobacterium tuberculosis direct test (MTD), and the results were compared with those of the conventional methods of fluorescence microscopy and cultivation (solid and radiometric media). One series of specimens (n = 515) was decontaminated with N-acetyl-L-cysteine (NALC)-NaOH: the other one (n = 423) was decontaminated with sodium dodecyl (lauryl) sulfate (SDS)-NaOH. Of the specimens decontaminated with NALC, 39 were MTD and culture positive, 455 were MTD and culture negative, 18 were MTD positive and culture negative, and 3 were MTD negative and culture positive, indicating a sensitivity of 92.9% and a specificity of 96.2% for the MTD. Of the specimens decontaminated with SDS, 35 were MTD and culture positive, 372 were MTD and culture negative, 15 were MTD positive and culture negative, and 1 was MTD negative and culture positive, indicating a sensitivity of 97.2% and a specificity of 96.1% for the MTD. After resolution of discrepant results by review of the patients' clinical data, the sensitivity of the MTD was 93.9%, the specificity was 97.6%, the positive predictive value was 80.7%, and the negative predictive value was 99.3% for the NALC series; the corresponding values were 97.4, 96.9, 76.0, and 99.7%, respectively, for the SDS series. In conclusion, the MTD is a highly sensitive and specific technique for detecting M. tuberculosis complex within hours in both smear-positive and smear-negative respiratory specimens.
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