ArticleThe Journal of cell biology1994
Determination of four biochemically distinct, sequential stages during vacuole inheritance in vitro.
Article in The Journal of cell biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
35 citing papers in PubMed, 75 citations in OpenAlex.
- Article
- Vacuolar SNARE protein transmembrane domains serve as nonspecific membrane anchors with unequal roles in lipid mixing.The Journal of biological chemistry · 2015Article
- Dissecting functions of the conserved oligomeric Golgi tethering complex using a cell-free assay.Traffic (Copenhagen, Denmark) · 2014Article
- Homotypic vacuole fusion in yeast requires organelle acidification and not the V-ATPase membrane domain.Developmental cell · 2013Article
- Cell-free reconstitution of vacuole membrane fragmentation reveals regulation of vacuole size and number by TORC1.Molecular biology of the cell · 2012Article
- Article
- Natamycin inhibits vacuole fusion at the priming phase via a specific interaction with ergosterol.Antimicrobial agents and chemotherapy · 2010Article
- Vacuole inheritance regulates cell size and branching frequency of Candida albicans hyphae.Molecular microbiology · 2009Article
- Assays of vacuole fusion resolve the stages of docking, lipid mixing, and content mixing.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Trans-SNARE interactions elicit Ca2+ efflux from the yeast vacuole lumen.The Journal of cell biology · 2004Article
- Vacuole membrane fusion: V0 functions after trans-SNARE pairing and is coupled to the Ca2+-releasing channel.The Journal of cell biology · 2003Article
- In vitro fusion between Saccharomyces cerevisiae secretory vesicles and cytoplasmic-side-out plasma membrane vesicles.The Biochemical journal · 2003Article
- Yeast vacuoles and membrane fusion pathways.The EMBO journal · 2002Review
- The Vtc proteins in vacuole fusion: coupling NSF activity to V(0) trans-complex formation.The EMBO journal · 2002Article
- Cvt18/Gsa12 is required for cytoplasm-to-vacuole transport, pexophagy, and autophagy in Saccharomyces cerevisiae and Pichia pastoris.Molecular biology of the cell · 2001Article
- Cdc42p functions at the docking stage of yeast vacuole membrane fusion.The EMBO journal · 2001Article
- Ergosterol is required for the Sec18/ATP-dependent priming step of homotypic vacuole fusion.The EMBO journal · 2001Article
- Mammalian inositol polyphosphate 5-phosphatase II can compensate for the absence of all three yeast Sac1-like-domain-containing 5-phosphatases.The Biochemical journal · 2001Article
- A Ypt/Rab effector complex containing the Sec1 homolog Vps33p is required for homotypic vacuole fusion.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Phosphatidylinositol 4,5-bisphosphate regulates two steps of homotypic vacuole fusion.Molecular biology of the cell · 2000Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Vacuole inheritance in Saccharomyces cerevisiae can be reconstituted in vitro using isolated organelles, cytosol, and ATP. Using the requirements of the reaction and its susceptibility to inhibitors, we have divided the in vitro reaction into four biochemically distinct, sequential subreactions. Stage I requires exposure of vacuoles to solutions of moderate ionic strength. Stage II requires "stage I" vacuoles and cytosol. In stage III, stage II vacuoles react with ATP. Finally, during stage IV, stage III vacuoles at a certain, minimal concentration complete the fusion reaction without further requirement for any soluble components. Reagents that inhibit the overall vacuole inheritance reaction block distinct stages. Stage III of the reaction is sensitive to the proton ionophore CCCP, to inhibitors of the vacuolar ATPase such as bafilomycin A1, and to the ATP-hydrolyzing enzyme apyrase, suggesting that an electrochemical potential across the vacuolar membrane is required during this stage. Inhibition studies with the amphiphilic peptide mastoparan and GTP gamma S suggest that GTP-hydrolyzing proteins might also be involved during this stage. Microcystin-LR, a specific inhibitor of protein phosphatases of type 1 and 2A, inhibits stage IV of the inheritance reaction, indicating that a protein dephosphorylation event is necessary for fusion. The definition of these four stages may allow the development of specific assays for the factors which catalyze each of the consecutive steps of the in vitro reaction.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.