ArticleProceedings of the National Academy of Sciences of the United States of America1994
Characterization of mutations induced by ethylnitrosourea in seminiferous tubule germ cells of transgenic B6C3F1 mice.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 94 citations in OpenAlex.
- A new mouse model of GLUT1 deficiency syndrome exhibits abnormal sleep-wake patterns and alterations of glucose kinetics in the brain.Disease models & mechanisms · 2019Article
- Recovery of a low mutant frequency after ionizing radiation-induced mutagenesis during spermatogenesis.Mutation research · 2008Article
- Article
- Article
- Mutagenicity testing with transgenic mice. Part II: Comparison with the mouse spot test.Journal of carcinogenesis · 2005Article
- Mutagenicity testing with transgenic mice. Part I: Comparison with the mouse bone marrow micronucleus test.Journal of carcinogenesis · 2005Article
- Spontaneous mutagenesis is enhanced in Apex heterozygous mice.Molecular and cellular biology · 2004Article
- Spontaneous hepatocellular carcinoma is reduced in transgenic mice overexpressing human O6- methylguanine-DNA methyltransferase.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Article
- Mutation frequency and specificity with age in liver, bladder and brain of lacI transgenic mice.Genetics · 2000Article
- Three new allelic mouse mutations that cause skeletal overgrowth involve the natriuretic peptide receptor C gene (Npr3).Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Review
- p53 deficiency does not affect the accumulation of point mutations in a transgene target.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
- Temporal and molecular characteristics of mutations induced by ethylnitrosourea in germ cells isolated from seminiferous tubules and in spermatozoa of lacZ transgenic mice.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
- Phenotypic and physical analysis of a chemically induced mutation disrupting anterior axial development in the mouse.Mammalian genome : official journal of the International Mammalian Genome Society · 1995Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Transgenic B6C3F1 mice carrying a lacI target gene were exposed to acute and multiple doses of ethylnitrosourea (ENU), and germ cells from the seminiferous tubules were assayed for mutation 3 and 90 days after treatment. Relative to untreated controls, the mutation frequency increased 3.2- and 19.9-fold at 3 and 90 days after treatment, respectively. Mutant lacI genes recovered from untreated and treated groups were sequenced, and the spectra of mutations were determined. Eighty-five percent (11/13) of the spontaneous mutations resulted in G.C-->A.T transitions, all of which occurred at CpG dinucleotides. Fifteen of 22 sites (68%) found mutated 3 days after ENU treatment occurred at G.C base pairs, although some of these are expected to be spontaneous mutations. Ninety days after treatment, 13 of 19 sites (68%) found mutated occurred at A.T base pairs. The mutation spectra seen are consistent with proposed mechanisms of ENU mutagenesis and correlate with the in vivo spectra seen in ENU studies by using transmissibility assays and the hprt gene. These findings represent significant progress toward defining the in vivo spectra of ENU mutagenesis in mammalian germ cells.
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