ArticleMolecular biology of the cell1994
Cytosolic ARFs are required for vesicle formation but not for cell-free intra-Golgi transport: evidence for coated vesicle-independent transport.
Article in Molecular biology of the cell, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 51 citations in OpenAlex.
- Poliovirus proteins induce membrane association of GTPase ADP-ribosylation factor.Journal of virology · 2005Article
- ER/Golgi intermediates acquire Golgi enzymes by brefeldin A-sensitive retrograde transport in vitro.The Journal of cell biology · 1999Article
- Roles for alpha(2)p24 and COPI in endoplasmic reticulum cargo exit site formation.The Journal of cell biology · 1999Article
- Fusogenic domains of golgi membranes are sequestered into specialized regions of the stack that can be released by mechanical fragmentation.The Journal of cell biology · 1999Article
- The molecular characterization of transport vesicles.Plant molecular biology · 1998Review
- ADP ribosylation factor regulates spectrin binding to the Golgi complex.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Cell-free transport to distinct Golgi cisternae is compartment specific and ARF independent.The Journal of cell biology · 1998Article
- Isolation of functional Golgi-derived vesicles with a possible role in retrograde transport.The Journal of cell biology · 1998Article
- Variations on the intracellular transport theme: maturing cisternae and trafficking tubules.The Journal of cell biology · 1997Review
- COPII vesicles derived from mammalian endoplasmic reticulum microsomes recruit COPI.The Journal of cell biology · 1996Article
- Human ARF4 expression rescues sec7 mutant yeast cells.Molecular and cellular biology · 1996Article
- Mass-spectrometric analysis of ADP-ribosylation factors from bovine brain: identification and evidence for homogeneous acylation with the C14:0 fatty acid (myristate).The Biochemical journal · 1995Article
- p115 is a general vesicular transport factor related to the yeast endoplasmic reticulum to Golgi transport factor Uso1p.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
- The G protein-activating peptide, mastoparan, and the synthetic NH2-terminal ARF peptide, ARFp13, inhibit in vitro Golgi transport by irreversibly damaging membranes.The Journal of cell biology · 1994Article
- Isolation and characterization of mutant CHO cell lines with compartment-specific resistance to brefeldin A.The Journal of cell biology · 1994Article
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4 authors at 1 institution in 1 country.
Funding
Abstract
We investigated the role of ADP-ribosylation factors (ARFs) in Golgi function using biochemical and morphological cell-free assays. An ARF-free cytosol produced from soluble Chinese hamster ovary (CHO) extracts supports intra-Golgi transport by a mechanism that is biochemically indistinguishable from control transport reactions: ARF-free transport reactions are NSF-dependent, remain sensitive to the donor Golgi-specific inhibitor ilimaquinone, and exhibit kinetics that are identical to that of control reactions containing ARFs. In contrast, ARF-free cytosol does not support the formation of coated vesicles on Golgi cisternae. However, vesicle formation is reconstituted upon the addition of ARF1. These data suggest that neither soluble ARFs nor coated vesicle formation are essential for transport. We conclude that cell-free intra-Golgi transport proceeds via a coated vesicle-independent mechanism regardless of vesicle formation on Golgi cisternae.
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