ArticlePlant molecular biology1994
The 24 kDa outer envelope membrane protein from spinach chloroplasts: molecular cloning, in vivo expression and import pathway of a protein with unusual properties.
Article in Plant molecular biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 47 citations in OpenAlex.
- Pepper mild mottle virus coat protein interacts with pepper chloroplast outer envelope membrane protein OMP24 to inhibit antiviral immunity in plants.Horticulture research · 2023Article
- The C-terminus of Bienertia sinuspersici Toc159 contains essential elements for its targeting and anchorage to the chloroplast outer membrane.Frontiers in plant science · 2014Article
- The Plastid Outer Envelope - A Highly Dynamic Interface between Plastid and Cytoplasm.Frontiers in plant science · 2011Article
- Development-specific association of amyloplasts with microtubules in scale cells of Narcissus tazetta.Protoplasma · 2007Article
- Mutation of the TGD1 chloroplast envelope protein affects phosphatidate metabolism in Arabidopsis.The Plant cell · 2005Article
- Import pathways of chloroplast interior proteins and the outer-membrane protein OEP14 converge at Toc75.The Plant cell · 2004Article
- Tomato allene oxide synthase and fatty acid hydroperoxide lyase, two cytochrome P450s involved in oxylipin metabolism, are targeted to different membranes of chloroplast envelope.Plant physiology · 2001Article
- Insertion of OEP14 into the outer envelope membrane is mediated by proteinaceous components of chloroplasts.The Plant cell · 2000Article
- Protein translocation into and across the chloroplastic envelope membranes.Plant molecular biology · 1998Review
- The role of lipids in plastid protein transport.Plant molecular biology · 1998Review
- Positive charges determine the topology and functionality of the transmembrane domain in the chloroplastic outer envelope protein Toc34.The Journal of cell biology · 1998Article
- Protein targeting and integration signal for the chloroplastic outer envelope membrane.The Plant cell · 1996Article
- A novel, bipartite transit peptide targets OEP75 to the outer membrane of the chloroplastic envelope.The Plant cell · 1996Article
- The N-terminal hydrophobic region of the mature phosphate translocator is sufficient for targeting to the chloroplast inner envelope membrane.The Plant cell · 1995Article
- A component of the chloroplastic protein import apparatus is targeted to the outer envelope membrane via a novel pathway.The EMBO journal · 1995Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The 24 kDa outer envelope membrane protein of spinach chloroplasts (omp24) represents a major constituent of this membrane. Sequences of tryptic and endoprotease Glu-C peptides derived from omp24 allowed the design of oligonucleotides which were used to generate a DNA fragment by polymerase chain reaction using spinach cDNA as template. This fragment served as a probe to screen a cDNA library for a full-length clone of the omp24 coding sequence. The protein predicted from the complete sequence only has 148 amino acids and a molecular mass of 16294 Da. It is an acidic protein (calculated isoelectric point 4.8) with a high content of proline residues. Expression of the coding sequence in Escherichia coli and characterization of the purified recombinant protein produced revealed that the overestimation of its molecular mass by SDS-PAGE (ca. 25 kDa) is due to its abnormal amino acid composition. Despite its rather low hydrophobicity (polarity index 49%), omp24 appears to be deeply embedded in the outer membrane. Insertion of omp24 into the membrane proceeds almost independently of surface receptors or targeting sequence but, in contrast to other known outer envelope membrane proteins, is stimulated by ATP.
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