ArticleThe Journal of physiology1994
Activation properties of chemically skinned fibres from human isolated bronchial smooth muscle.
Article in The Journal of physiology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
4 citing papers in PubMed, 31 citations in OpenAlex.
- The pivotal role of airway smooth muscle in asthma pathophysiology.Journal of allergy · 2011Article
- Mechanisms of hydralazine induced vasodilation in rabbit aorta and pulmonary artery.British journal of pharmacology · 2001Article
- Control of pulmonary vascular smooth muscle tone by sarcoplasmic reticulum Ca2+ pump blockers: thapsigargin and cyclopiazonic acid.Pflugers Archiv : European journal of physiology · 1995Article
- Diosmin-induced increase in sensitivity to Ca2+ of the smooth muscle contractile apparatus in the rat isolated femoral vein.British journal of pharmacology · 1994Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
1. The contractile activation properties of human isolated bronchial smooth muscle were investigated using chemically (beta-escin) skinned strips. 2. Concentration-dependent contractions were induced by free ion concentrations of Ca2+ (0.5-3 microM), Sr2+ (2-200 microM) and Ba2+ (50-1000 microM). The resulting -log[cation]-tension relationships were fitted by sigmoidal curves with EC50 values (cation concentration required to produce half-maximal tension) and co-operativity factors (Hill coefficient, nH) of, respectively, 0.25 microM and 3.4 for Ca2+, 12 microM and 2.64 for Sr2+ and 100 microM and 1.73 for Ba2+. Maximal responses to Sr2+ and Ba2+ were 125.5 +/- 15.4 and 96 +/- 8.1% (n = 5) respectively of the maximum tension induced by Ca2+. 3. Trifluoperazine (5-100 microM), cyclic AMP (50-300 microM) and cyclic GMP (50-100 microM) each antagonized Ca2+ in a concentration-dependent manner. On the other hand, okadaic acid (OA, 0.2-1 microM) potentiated Ca2+ and increased the maximum response to Ca2+ (+25 +/- 5.4%, n = 5, for 1 microM OA). 4. This study has demonstrated the high Ca2+ sensitivity of the activation mechanism of human isolated bronchial smooth muscle. It also suggests that control of the contractile machinery in the human bronchus involves processes of phosphorylation and dephosphorylation. The beta-escin-treated human bronchus may be a useful model for investigating the cellular basis of some pathophysiological processes such as bronchial hyper-responsiveness.
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