ArticleThe EMBO journal1994
Double-pulse facilitation of smooth muscle alpha 1-subunit Ca2+ channels expressed in CHO cells.
Article in The EMBO journal, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 55 citations in OpenAlex.
- Regulation of Cardiac Cav1.2 Channels by Calmodulin.International journal of molecular sciences · 2023Review
- Facilitation of murine cardiac L-type Ca(v)1.2 channel is modulated by calmodulin kinase II-dependent phosphorylation of S1512 and S1570.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Beta subunits of voltage-gated calcium channels.Journal of bioenergetics and biomembranes · 2003Review
- The beta1a subunit regulates the functional properties of adult frog and mouse L-type Ca2+ channels of skeletal muscle.The Journal of physiology · 2002Article
- Potentiation of the cardiac L-type Ca(2+) channel (alpha(1C)) by dihydropyridine agonist and strong depolarization occur via distinct mechanisms.The Journal of general physiology · 2001Article
- Modulation of the Ca(2+)-induced Ca(2+) release cascade by beta-adrenergic stimulation in rat ventricular myocytes.The Journal of physiology · 2001Article
- The alpha 1-subunit of smooth muscle Ca(2+) channel preserves multiple open states induced by depolarization.The Journal of physiology · 2000Article
- Anomalous L-type calcium channels of rat spinal motoneurons.The Journal of general physiology · 1999Article
- Structures and functions of calcium channel beta subunits.Journal of bioenergetics and biomembranes · 1998Review
- Overview of voltage-dependent calcium channels.Journal of bioenergetics and biomembranes · 1998Review
- cAMP-dependent enhancement of dihydropyridine-sensitive calcium channel availability in hippocampal neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1997Article
- Multiple components of Ca2+ channel facilitation in cerebellar granule cells: expression of facilitation during development in culture.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1996Article
- Coexpression of the beta2 subunit does not induce voltage-dependent facilitation of the class C L-type Ca channel.Pflugers Archiv : European journal of physiology · 1996Article
- On the regulation of the expressed L-type calcium channel by cAMP-dependent phosphorylation.Pflugers Archiv : European journal of physiology · 1995Article
- Recruitment of plasma membrane voltage-dependent calcium-permeable channels in carrot cells.The EMBO journal · 1994Article
- Voltage-dependent facilitation of a neuronal alpha 1C L-type calcium channel.The EMBO journal · 1994Article
- Functional properties of the CaV1.2 calcium channel activated by calmodulin in the absence of alpha2delta subunits.Channels (Austin, Tex.)Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Frequent strong depolarizations facilitate Ca2+ channels in various cell types by shifting their gating behavior towards mode 2, which is characterized by long openings and high probability of being open. In cardiac cells, the same type of gating behavior is potentiated by beta-adrenoceptors presumably acting via phosphorylation of a protein identical to or associated with the channel. Voltage-dependent phosphorylation has also been reported to underlie Ca2+ channel facilitation in chromaffin adrenal medulla and in skeletal muscle cells. We studied a possible voltage-dependent facilitation of the principal channel forming alpha 1-subunit of the dihydropyridine-sensitive smooth muscle Ca2+ channel. Single channel and whole-cell Ca2+ currents were recorded in Chinese hamster ovary cells stably expressing the class Cb Ca2+ channel alpha 1-subunit. Strong depolarizing voltage-clamp steps preceding the test pulse resulted in a 2- to 3-fold increase of the single Ca2+ channel activity and induction of mode 2-like gating behavior. Accordingly we observed a significant potentiation of the whole-cell current by approximately 50%. In contrast to the previous suggestions we found no experimental evidence for involvement of channel phosphorylation by protein kinases (cAMP-dependent protein kinase, protein kinase C and other protein kinases utilizing ATP gamma S) in the control and facilitated current. The data demonstrate that the L-type Ca2+ channel alpha 1-subunit solely expressed in Chinese hamster ovary cells is subject to a voltage-dependent facilitation but not to phosphorylation.(ABSTRACT TRUNCATED AT 250 WORDS)
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