ArticleIn vitro cellular & developmental biology. Animal1994
Defined medium for primary culture de novo of adult rat alveolar epithelial cells.
Article in In vitro cellular & developmental biology. Animal, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 68 citations in OpenAlex.
- Characteristics of Passive Solute Transport across Primary Rat Alveolar Epithelial Cell Monolayers.Membranes · 2021Article
- Cross-Species Transcriptome Profiling Identifies New Alveolar Epithelial Type I Cell-Specific Genes.American journal of respiratory cell and molecular biology · 2017Article
- p300/β-Catenin Interactions Regulate Adult Progenitor Cell Differentiation Downstream of WNT5a/Protein Kinase C (PKC).The Journal of biological chemistry · 2016Article
- Local influence of cell viability on stretch-induced permeability of alveolar epithelial cell monolayers.Cellular and molecular bioengineering · 2016Article
- Combinations of differentiation markers distinguish subpopulations of alveolar epithelial cells in adult lung.American journal of physiology. Lung cellular and molecular physiology · 2016Article
- Transforming Growth Factor-β1 Induced Epithelial Mesenchymal Transition is blocked by a chemical antagonist of translation factor eIF4E.Scientific reports · 2015Article
- Enolase 1 (ENO1) and protein disulfide-isomerase associated 3 (PDIA3) regulate Wnt/β-catenin-driven trans-differentiation of murine alveolar epithelial cells.Disease models & mechanisms · 2015Article
- Alveolar epithelium: beyond the barrier.American journal of respiratory cell and molecular biology · 2014Article
- Transdifferentiation of alveolar epithelial type II to type I cells is controlled by opposing TGF-β and BMP signaling.American journal of physiology. Lung cellular and molecular physiology · 2013Article
- Rac1 pathway mediates stretch response in pulmonary alveolar epithelial cells.American journal of physiology. Lung cellular and molecular physiology · 2013Article
- Rho kinase signaling pathways during stretch in primary alveolar epithelia.American journal of physiology. Lung cellular and molecular physiology · 2012Article
- Endothelial-monocyte activating polypeptide II disrupts alveolar epithelial type II to type I cell transdifferentiation.Respiratory research · 2012Article
- Troglitazone attenuates TGF-β1-induced EMT in alveolar epithelial cells via a PPARγ-independent mechanism.PloS one · 2012Article
- Translocation of PEGylated quantum dots across rat alveolar epithelial cell monolayers.International journal of nanomedicine · 2011Article
- Alveolar epithelial cell injury due to zinc oxide nanoparticle exposure.American journal of respiratory and critical care medicine · 2010Article
- Stretch magnitude and frequency-dependent actin cytoskeleton remodeling in alveolar epithelia.American journal of physiology. Cell physiology · 2010Article
- Over-expression of caveolin-1 aggravate LPS-induced inflammatory response in AT-1 cells via up-regulation of cPLA2/p38 MAPK.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2010Article
- Article
- Mechanisms of alveolar epithelial translocation of a defined population of nanoparticles.American journal of respiratory cell and molecular biology · 2010Article
- N-acetylcysteine inhibits alveolar epithelial-mesenchymal transition.American journal of physiology. Lung cellular and molecular physiology · 2009Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Isolated type II pneumocytes grown in serum on tissue culture-treated polycarbonate filters form monolayers with characteristic bioelectric properties, and change morphologically with time in culture to resemble type I cells. Concurrently, the cells express type I cell surface epitopes, making this a potentially useful in vitro model with which to study regulation of alveolar epithelial cell function and differentiation. To define specific soluble growth factors and matrix substances that may regulate these processes, it would be preferable to culture isolated pneumocytes de novo under completely defined, serum-free conditions. In this study, we developed a completely defined serum-free medium that is capable of supporting alveolar epithelial cells in primary culture, allowing the formation of monolayers with characteristic bioelectric and phenotypic properties. Freshly isolated rat type II cells were resuspended in completely defined serum-free medium and plated de novo on polycarbonate filters. Plating efficiency, bioelectric properties, morphology, and binding of a type I cell-specific monoclonal antibody were determined as functions of time. Plating efficiency plateaus at about 14% by Day 3 in culture. Transepithelial resistance rises to high levels, peaking at 1.76 +/- 0.14 K omega-cm2 by Day 5 in culture. Short-circuit current peaks on Day 3 in culture at 2.71 +/- 0.35 microA/cm2. With time, the cells gradually become flattened with protuberant nuclei and long cytoplasmic extensions, more closely resembling type I cells, and begin to express a type I cell surface epitope.(ABSTRACT TRUNCATED AT 250 WORDS)
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.