ArticleBiophysical journal1994
Cross-linker dynamics determine the mechanical properties of actin gels.
Article in Biophysical journal, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 85 papers.
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Who cites it
85 citing papers in PubMed, 247 citations in OpenAlex.
- Rethinking contractility in active cytoskeletal matter.Biophysical journal · 2026Article
- Crosslinker identity and dynamics regulate F-actin network structure and mechanics.Frontiers in cell and developmental biology · 2026Article
- Myosin-I synergizes with Arp2/3 complex to enhance the pushing forces of branched actin networks.Science advances · 2024Article
- Defective Biomechanics and Pharmacological Rescue of Human Cardiomyocytes with Filamin C Truncations.International journal of molecular sciences · 2024Article
- Mechanosensitivity of phase separation in an elastic gel.The European physical journal. E, Soft matter · 2024Article
- Myosin-I Synergizes with Arp2/3 Complex to Enhance Pushing Forces of Branched Actin Networks.bioRxiv : the preprint server for biology · 2024Article
- Highly flexible PEG-LifeAct constructs act as tunable biomimetic actin crosslinkers.Soft matter · 2024Article
- From stress fiber to focal adhesion: a role of actin crosslinkers in force transmission.Frontiers in cell and developmental biology · 2024Review
- Actin crosslinking by α-actinin averts viscous dissipation of myosin force transmission in stress fibers.iScience · 2023Article
- Network dynamics of the nonlinear power-law relaxation of cell cortex.Biophysical journal · 2022Article
- The effects of whey protein fibrils on the linear and non-linear rheological properties of a gluten-free dough.Frontiers in nutrition · 2022Article
- Simulations of dynamically cross-linked actin networks: Morphology, rheology, and hydrodynamic interactions.PLoS computational biology · 2021Article
- Elastic wrinkling of keratocyte lamellipodia driven by myosin-induced contractile stress.Biophysical journal · 2021Article
- Calcium modulates the domain flexibility and function of an α-actinin similar to the ancestral α-actinin.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Dynamic actin cross-linking governs the cytoplasm's transition to fluid-like behavior.Molecular biology of the cell · 2020Article
- Tug-of-war between actomyosin-driven antagonistic forces determines the positioning symmetry in cell-sized confinement.Nature communications · 2020Article
- Cell response to substrate rigidity is regulated by active and passive cytoskeletal stress.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Non-monotonic dependence of stiffness on actin crosslinking in cytoskeleton composites.Soft matter · 2019Article
- Quantifying the Local Mechanical Properties of Cells in a Fibrous Three-Dimensional Microenvironment.Biophysical journal · 2019Article
- Actin assembly produces sufficient forces for endocytosis in yeast.Molecular biology of the cell · 2019Article
25 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
To evaluate the contributions of cross-linker dynamics and polymer deformation to the frequency-dependent stiffness of actin filament gels, we compared the rheological properties of actin gels with three types of cross-linkers: a weak one, Acanthamoeba alpha-actinin (dissociation rate constant 5.2 s-1, association rate constant 1.1 x 10(6) M-1 s-1); a strong one, chicken smooth muscle alpha-actinin (dissociation rate constant 0.66 s-1, association rate constant 1.20 x 10(6) M-1 s-1); and an extremely strong one, biotin/avidin (dissociation rate constant approximately zero). The biotin/avidin cross-linked gel, whose behavior is determined by polymer bending alone, behaves like a solid and shows no frequency dependence. The amoeba alpha-actinin cross-linked gel behaves like a viscoelastic fluid, and the frequency dependence of the stiffness can be explained by a mathematical model for dynamically cross-linked gels. The stiffness of the chicken alpha-actinin cross-linked gel is independent of frequency, and has viscoelastic properties intermediate between the two. The two alpha-actinins have similar association rate constants for binding to actin filaments, consistent with a diffusion-limited reaction. Rigid cross-links make the gel stiff, but make it elastic without the ability to deform permanently. Dynamically cross-linked actin filaments should allow the cell to react passively to various outside forces without any sort of signaling. Slower, signal-mediated pathways, such as severing filaments or changing the affinity of cross-linkers, could alter the nature of these passive reactions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.