ArticleThe Journal of physiology1994
K+ and Cl- transport mechanisms in bovine pigment epithelium that could modulate subretinal space volume and composition.
Article in The Journal of physiology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 103 citations in OpenAlex.
- Kv2.1/Kv8.2 Channels Regulate Fluid Homeostasis in the Outer Retina.bioRxiv : the preprint server for biology · 2026Article
- Review of the clinical electrooculogram - Part 1: Mechanism of the Light-Rise.Documenta ophthalmologica. Advances in ophthalmology · 2026Review
- Membrane-resolved epithelial electrophysiology revealed using extracellular electrochemical impedance spectroscopy (EEIS).bioRxiv : the preprint server for biology · 2026Article
- Diuretic inhibition of experimental myopia implicates retinal ion-driven efflux in the regulation of ocular growth.Frontiers in medicine · 2026Article
- On the physiological processes underlying optoretinography [Invited].Biomedical optics express · 2025Review
- Extracellular Matrix (ECM) Aging in the Retina: The Role of Matrix Metalloproteinases (MMPs) in Bruch's Membrane Pathology and Age-Related Macular Degeneration (AMD).Biomolecules · 2025Review
- pH in the vertebrate retina and its naturally occurring and pathological changes.Progress in retinal and eye research · 2025Review
- A biologically validated mathematical model for decoding epithelial apical, basolateral, and paracellular electrical properties.American journal of physiology. Cell physiology · 2023Article
- Article
- Changes in transepithelial electrical resistance and intracellular ion concentration in TGF-β-induced epithelial-mesenchymal transition of retinal pigment epithelial cells.American journal of translational research · 2022Article
- SLC26A7 constitutes the thiocyanate-selective anion conductance of the basolateral membrane of the retinal pigment epithelium.American journal of physiology. Cell physiology · 2020Article
- Mechanisms of Ion Transport Across the Mouse Retinal Pigment Epithelium Measured In Vitro.Investigative ophthalmology & visual science · 2020Article
- Fluid and solute transport across the retinal pigment epithelium: a theoretical model.Journal of the Royal Society, Interface · 2020Article
- High-yield, automated intracellular electrophysiology in retinal pigment epithelia.Journal of neuroscience methods · 2019Article
- The logic of ionic homeostasis: Cations are for voltage, but not for volume.PLoS computational biology · 2019Article
- Article
- Mouse retinal pigment epithelial cells exhibit a thiocyanate-selective conductance.American journal of physiology. Cell physiology · 2018Article
- Article
- Oxytocin (OXT)-stimulated inhibition of Kir7.1 activity is through PIPCellular signalling · 2017Article
- In Pursuit of Authenticity: Induced Pluripotent Stem Cell-Derived Retinal Pigment Epithelium for Clinical Applications.Stem cells translational medicine · 2016Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
1. Conventional and ion-selective double-barrelled microelectrodes were used in an in vitro bovine retinal pigment epithelium (RPE)-choroid preparation to measure the changes in membrane voltage, resistance and intracellular K+ and Cl- activities produced by small, physiological changes in extracellular potassium ([K+]o). 2. In the intact eye, light-induced changes in [K+]o occur in the extracellular (or subretinal) space that separates the neural retina and the RPE apical membrane. These [K+]o changes can be approximated in vitro by decreasing apical bath [K+]o from 5 to 2 mM. 3. This in vitro change in [K+]o simultaneously decreased intracellular Cl- and K+ activities (aCli and aKi) by 25 +/- 6 mM (n = 8) and 19 +/- 7 mM (n = 4) (mean +/- S.D.), respectively. In control Ringer solution (5 mM [K+]o) aCli and aKi were 65 +/- 10 mM (n = 28) and 65 +/- 8 mM (n = 6), respectively. 4. The [K+]o-induced decreases in aCli and aKi were both significantly inhibited, either by blocking the apical membrane K+ conductance with Ba2+ or the basolateral membrane Cl- conductance with DIDS (4,4'-diisothiocyano-stilbene-2,2'-disulphonic acid). 5. Transepithelial current pulses were used to determine the relative basolateral membrane Cl- conductance, TClBAS, was approximately 0.6 (n = 3), and the relative apical membrane K+ conductance, TKAP, was approximately 0.7 (n = 2). Step changes in basal bath [K+]o were used to estimate the relative basolateral membrane K+ conductance, TKBAS, was approximately 0.34 (n = 3). 6. These data show that the apical membrane K+ conductance and the basolateral membrane Cl- conductance are electrically coupled. In vivo, this coupling could have significant functional importance by modulating the relative hydration of the subretinal space, regulating RPE cell volume, and buffering the chemical composition of the subretinal space.
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