ArticleThe Journal of experimental medicine1994
Differential activation of transcription versus recombination of transgenic T cell receptor beta variable region gene segments in B and T lineage cells.
Article in The Journal of experimental medicine, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 83 citations in OpenAlex.
- Lineage- and stage-specific activity of antigen receptor gene enhancers during lymphocyte development.Frontiers in epigenetics and epigenomics · 2024Review
- Recombination may occur in the absence of transcription in the immunoglobulin heavy chain recombination centre.Nucleic acids research · 2020Article
- The epigenetic profile of Ig genes is dynamically regulated during B cell differentiation and is modulated by pre-B cell receptor signaling.Journal of immunology (Baltimore, Md. : 1950) · 2009Article
- Activation of 12/23-RSS-dependent RAG cleavage by hSWI/SNF complex in the absence of transcription.Molecular cell · 2008Article
- Productive coupling of accessible Vbeta14 segments and DJbeta complexes determines the frequency of Vbeta14 rearrangement.Journal of immunology (Baltimore, Md. : 1950) · 2008Article
- SMAR1 and Cux/CDP modulate chromatin and act as negative regulators of the TCRbeta enhancer (Ebeta).Nucleic acids research · 2004Article
- T cell receptor (TCR) alpha/delta locus enhancer identity and position are critical for the assembly of TCR delta and alpha variable region genes.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Pax5 is required for recombination of transcribed, acetylated, 5' IgH V gene segments.Genes & development · 2003Article
- Insertion of phosphoglycerine kinase (PGK)-neo 5' of Jlambda1 dramatically enhances VJlambda1 rearrangement.The Journal of experimental medicine · 2001Article
- V(D)J recombination is not activated by demethylation of the kappa locus.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Mechanisms that direct ordered assembly of T cell receptor beta locus V, D, and J gene segments.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Definition of a T-cell receptor beta gene core enhancer of V(D)J recombination by transgenic mapping.Molecular and cellular biology · 2000Article
- Chromatin remodeling directly activates V(D)J recombination.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- A targeted deletion of a region upstream from the Jkappa cluster impairs kappa chain rearrangement in cis in mice and in the 103/bcl2 cell line.The Journal of experimental medicine · 1999Article
- Regulation of V(D)J recombination by transcriptional promoters.Molecular and cellular biology · 1999Article
- Recombination and transcription of the endogenous Ig heavy chain locus is effected by the Ig heavy chain intronic enhancer core region in the absence of the matrix attachment regions.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Positive and negative regulation of V(D)J recombination by the E2A proteins.The Journal of experimental medicine · 1999Article
- Evidence that immunoglobulin VH-DJ recombination does not require germ line transcription of the recombining variable gene segment.Molecular and cellular biology · 1998Article
- Kappa chain monoallelic demethylation and the establishment of allelic exclusion.Genes & development · 1998Article
- Enhancer control of local accessibility to V(D)J recombinase.Molecular and cellular biology · 1997Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
We have tested the ability of the T cell receptor beta (TCR-beta) transcriptional enhancer (E beta) to confer transcriptional activation and tissue-specific V(D)J recombination of TCR-beta V, D, and J segments in a transgenic minilocus recombination substrate. We find that the minimal E beta element, as previously shown for a DNA segment that contained the E mu element, promotes a high level of substrate D to J beta rearrangement in both B and T cells, but only promotes V beta to DJ beta rearrangement in T cells. Thus, both the E mu and E beta elements similarly direct V(D)J recombination of this substrate in vivo, supporting a general role for transcriptional enhancers in the normal regulation of this rearrangement process. Surprisingly, however, we found that both the V beta and DJ beta portion of the constructs were transcribed in an enhancer-dependent fashion (conferred by either E mu or E beta) in both B and T lineage cells, including normal precursor B cells propagated in culture. These findings indicate that, at least in some contexts, transcriptional activation, per se, is not sufficient to confer V(D)J recombinational accessibility to a substrate V gene segment.
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