Evidence map›Paper›PMID 8002562›Full record

ArticleJournal of bacteriology1994

Physical and genetic map of Streptococcus thermophilus A054.

Y Roussel, M Pebay, G Guedon, J M Simonet, B Decaris

Abstract read
In one paragraph

Article in Journal of bacteriology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Genomic organization of lactic acid bacteria.Antonie van Leeuwenhoek · 1996
    Review
  5. Article
  6. Article
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Y RousselLaboratoire de Génétique et Microbiologie, Université Henri Poincaré Nancy I, France.
M Pebay
G Guedon
J M Simonet
B Decaris

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The three restriction endonucleases SfiI, BssHII, and SmaI were found to generate fragments with suitable size distributions for mapping the genome of Streptococcus thermophilus A054. A total of 5, 8, and 24 fragments were produced with SfiI, BssHII, and SmaI, respectively. An average genome size of 1,824 kb was determined by summing the total fragment sizes obtained by digestions with these three enzymes. Partial and multiple digestions of genomic DNA in conjunction with Southern hybridization were used to map SfiI, BssHII, and SmaI fragments. All restriction fragments were arranged in a unique circular chromosome. Southern hybridization analysis with specific probes allowed 23 genetic markers to be located on the restriction map. Among them, six rrn loci were precisely located. The area of the chromosome containing the ribosomal operons was further detailed by mapping some of the ApaI and SgrAI sites. Comparison of macrorestriction patterns from three clones derived from strain A054 revealed two variable regions in the chromosome. One was associated with the tandem rrnD and rrnE loci, and the other was mapped in the region of the lactose operon.

Indexed as

Genome, BacterialRestriction MappingBlotting, SouthernChromosomes, BacterialDeoxyribonucleases, Type II Site-SpecificDNA, BacterialElectrophoresis, Gel, Pulsed-FieldGenetic MarkersGenetic VariationSpecies SpecificityStreptococcusCCCGGG-specific type II deoxyribonucleasesDeoxyribonucleases, Type II Site-SpecificDNA, Bacterialendodeoxyribonuclease BSSHIIendodeoxyribonuclease SfiIGenetic Markers

Identifiers

PMID8002562
PMCPMC197195

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.