Evidence map›Paper›PMID 8001165›Full record

ArticleCurrent genetics1994

A luciferase expression system for Physarum that facilitates analysis of regulatory elements.

J Bailey, M Benard, T G Burland

Abstract readComparative Study
PubMed Publisher
In one paragraph

Article in Current genetics, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
2.1field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 14 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 3 institutions in 3 countries.

J BaileyGenetics Department, University of Leicester, UK.
M Benard
T G Burland
Centre National de la Recherche Scientifique · FRUniversity of Leicester · GBUniversity of Wisconsin–Madison · US

Funding

STOREROOM AND MATERIALS ACQUISITION LABORATORYP30CA007175 · NCI · UNIVERSITY OF WISCONSIN MADISON · PI DRINKWATER, NORMAN R. · 1985 to 2000
$2.4M
THE CELL CYCLE &DIFFERENTIATION IN TUMOR BIOLOGYP01CA023076 · NCI · UNIVERSITY OF WISCONSIN MADISON · PI DOVE, WILLIAM FRANKLIN · 1985 to 1992
–
NCI NIH HHS CA07175NCI NIH HHS CA23076Wellcome Trust
6 · The paper itself

Abstract

We have developed a transient expression system for the protist Physarum polycephalum based on firefly luciferase. We demonstrate the utility of this system for comparing the activities of different promoters in Physarum amoebae, and also for detecting genetic elements that affect the level of gene expression. This system is likely to facilitate improvements in the stable transformation of this organism.

Indexed as

Promoter Regions, GeneticAnimalsCloning, MolecularColeopteraElectroporationGene ExpressionKineticsLuciferasesPhysarum polycephalumRecombinant ProteinsRestriction MappingLuciferasesRecombinant Proteins

Identifiers

PMID8001165
OpenAlexW2014134610

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.