ArticleCell and tissue research1994
Expression of vacuolar H(+)-ATPase in osteoclasts and its role in resorption.
Article in Cell and tissue research, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
5 citing papers in PubMed, 46 citations in OpenAlex.
- Enhanced osteoblast and osteoclast responses to a thin film sputtered hydroxyapatite coating.Journal of materials science. Materials in medicine · 2011Article
- Cell interactions in bone tissue engineering.Journal of cellular and molecular medicine · 2010Review
- Bovine dentine organic matrix down-regulates osteoclast activity.Journal of bone and mineral metabolism · 2009Article
- Reveromycin A, an agent for osteoporosis, inhibits bone resorption by inducing apoptosis specifically in osteoclasts.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- An ultrastructural evaluation of the effects of cysteine-proteinase inhibitors on osteoclastic resorptive functions.Calcified tissue international · 1995Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
By means of light- and electron-microscopic immunocytochemistry, we have demonstrated the expression of vacuolar H(+)-ATPase in mouse osteoclasts. In fully differentiated osteoclasts, intense immunolabeling was observed along the plasma membranes including those of ruffled borders and associated pale vesicles and vacuoles, whereas those of clear zones and basolateral cell surfaces were entirely free of immunoreaction. Specific expression of vacuolar H(+)-ATPase was also detected over polyribosomes and cisterns of the rough-surfaced endoplasmic reticulum. Multinucleated osteoclastic cells were suspended on dentine slices and cultured for 48 h in the presence or absence of either concanamycin B or bafilomycin A1, specific inhibitors of vacuolar H(+)-ATPase. Morphometric analysis of co-cultured dentine slices with backscattered electron microscopy revealed that both inhibitors strongly reduced the formation of resorption lacunae in a dose-dependent manner. These results suggest that vacuolar H(+)-ATPase is produced in the rough-surfaced endoplasmic reticulum, stored in the membrane vesicles, and transported into the ruffled border membranes of osteoclasts, and that this enzyme plays a key role in the creation of an acidic subosteoclastic microenvironment for the demineralization of co-cultured substrates.
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Registered trials
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