ArticleTransgenic research1994
Regulation of human protein C gene expression by the mouse WAP promoter.
Article in Transgenic research, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 12 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
12 citing papers in PubMed, 32 citations in OpenAlex.
- Secretion of human protein C in mouse milk.International journal of molecular sciences · 2015Article
- Analysis of lactation defects in transgenic mice.Journal of mammary gland biology and neoplasia · 2006Review
- A 470 bp WAP-promoter fragment confers lactation independent, progesterone regulated mammary-specific gene expression in transgenic mice.Transgenic research · 2005Article
- Transgenic mice expressing recombinant human protein C exhibit defects in lactation and impaired mammary gland development.Transgenic research · 2003Article
- Hormone-induced modifications of the chromatin structure surrounding upstream regulatory regions conserved between the mouse and rabbit whey acidic protein genes.The Biochemical journal · 2003Article
- A distal region, hypersensitive to DNase I, plays a key role in regulating rabbit whey acidic protein gene expression.The Biochemical journal · 2001Article
- A one-step gene amplification system for use in cultured mammalian cells and transgenic animals.Transgenic research · 2001Article
- Recombinant human protein C expression in the milk of transgenic pigs and the effect on endogenous milk immunoglobulin and transferrin levels.Transgenic research · 2001Article
- Production of recombinant human type I procollagen homotrimer in the mammary gland of transgenic mice.Transgenic research · 1999Article
- Expression of human erythropoietin transgenes and of the endogenous WAP gene in the mammary gland of transgenic rabbits during gestation and lactation.Transgenic research · 1998Article
- Proteolytic maturation of protein C upon engineering the mouse mammary gland to express furin.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
- Expression of genomic and cDNA transgenes after co-integration in transgenic mice.Transgenic research · 1995Article
Corrections and comments
- Erratum issued
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A 4.1 kb mouse whey acidic protein (mWAP) promoter was cloned from a C57BL/6 cosmid library. The tissue-specific and developmental pattern of expression of a hybrid gene comprised of the mWAP promoter fragment and the human protein C (HPC) gene was analysed in transgenic mice. The corresponding RNA was detected mainly in the mammary gland, with 'leakage' of expression in the salivary gland and kidney. The developmental pattern of transgene expression differed from that of the endogenous WAP gene. In particular, recombinant HPC (rHPC) transcripts were detected earlier in pregnancy than WAP RNA, with no significant increase during lactation. This indicates that regulatory elements responsible for developmental regulation are located outside the 4.1 kb mWAP gene promoter fragment, or if present, may be subject to position effects. Precocious expression of the transgene did not compromise the health or nursing abilities of transgenic females. Expression of rHPC affected the appearance of the mammary alveoli and alveolar epithelial cells in lactating transgenic mice. The alveoli were less distended and alveolar epithelial cells appeared cuboidal with centrally positioned nuclei. We suggest that the inefficient intracellular processing of rHPC can alter the histological appearance of alveolar epithelial cells in the transgenic mammary gland.
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