ArticleApplied and environmental microbiology1994
Engineering of Escherichia coli central metabolism for aromatic metabolite production with near theoretical yield.
Article in Applied and environmental microbiology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
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Who cites it
58 citing papers in PubMed, 206 citations in OpenAlex.
- Metabolic engineering of Corynebacterium crenatum for enhanced L-tyrosine production from mannitol and glucose.Microbial cell factories · 2024Article
- De novo biosynthesis of β-Arbutin in Komagataella phaffii based on metabolic engineering strategies.Microbial cell factories · 2024Article
- Increasing the robustness of Escherichia coli for aromatic chemicals production through transcription factor engineering.Advanced biotechnology · 2024Article
- A comprehensive review and comparison of L-tryptophan biosynthesis inFrontiers in bioengineering and biotechnology · 2023Review
- Production of indigo by recombinant bacteria.Bioresources and bioprocessing · 2023Review
- Improved production of the non-native cofactor FScientific reports · 2021Article
- Rational engineering of Kluyveromyces marxianus to create a chassis for the production of aromatic products.Microbial cell factories · 2020Article
- Combining mechanistic and machine learning models for predictive engineering and optimization of tryptophan metabolism.Nature communications · 2020Article
- Chromosome Engineering To Generate Plasmid-Free Phenylalanine- and Tyrosine-OverproducingApplied and environmental microbiology · 2020Article
- Bioprocess Optimization for the Production of Aromatic Compounds With Metabolically Engineered Hosts: Recent Developments and Future Challenges.Frontiers in bioengineering and biotechnology · 2020Review
- Two-Component-System RspA1/A2-Dependent Regulation on Primary Metabolism inFrontiers in microbiology · 2020Article
- Rewiring carbon metabolism in yeast for high level production of aromatic chemicals.Nature communications · 2019Article
- Metabolic engineering for the production of l-phenylalanine in3 Biotech · 2019Review
- Metabolic engineering of microorganisms for production of aromatic compounds.Microbial cell factories · 2019Review
- Metabolic engineering for improving L-tryptophan production in Escherichia coli.Journal of industrial microbiology & biotechnology · 2019Review
- Rational design and analysis of an Escherichia coli strain for high-efficiency tryptophan production.Journal of industrial microbiology & biotechnology · 2018Article
- Employing a biochemical protecting group for a sustainable indigo dyeing strategy.Nature chemical biology · 2018Article
- Engineering and systems-level analysis ofBiotechnology for biofuels · 2018Article
- Metabolic Engineering of the Shikimate Pathway for Production of Aromatics and Derived Compounds-Present and Future Strain Construction Strategies.Frontiers in bioengineering and biotechnology · 2018Article
- Genome mining of 2-phenylethanol biosynthetic genes fromBiotechnology for biofuels · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Escherichia coli and many other microorganisms synthesize aromatic amino acids through the condensation reaction between phosphoenolpyruvate (PEP) and erythrose 4-phosphate to form 3-deoxy-D-arabinoheptulosonate 7-phosphate (DAHP). It has been shown that overexpression of transketolase increases the production of DAHP in an aroB mutant strain (unable to further metabolize DAHP) with elevated DAHP synthase. However, the yield (percent conversion) of DAHP from glucose is still low. Stoichiometric analysis shows that many enzymes compete for intracellular PEP. In particular, the phosphotransferase system, responsible for glucose transport in E. coli, uses PEP as a phosphate donor and converts it to pyruvate, which is less likely to recycle back to PEP. This stoichiometric limitation greatly reduces the yield of aromatic metabolites. To relieve this limitation, we overexpressed PEP synthase in the presence of glucose and showed that it increased the final concentration and the yield of DAHP by almost twofold, to a near theoretical maximum. The PEP synthase effect is not observed without overproduced transketolase, suggesting that erythrose 4-phosphate is the first limiting metabolite. This result demonstrates the utility of pathway analysis and the limitation of central metabolites in the high-level overproduction of desired metabolites.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.