ArticleProceedings of the National Academy of Sciences of the United States of America1994
Targeted gene replacement demonstrates that myristoyl-CoA: protein N-myristoyltransferase is essential for viability of Cryptococcus neoformans.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
58 citing papers in PubMed, 170 citations in OpenAlex.
- Article
- Biolistic Transformation of Cryptococcus neoformans.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Article
- Molecular targets for antifungals in amino acid and protein biosynthetic pathways.Amino acids · 2021Review
- A Not-So-Ancient Grease History: Click Chemistry and Protein Lipid Modifications.Chemical reviews · 2021Review
- Genetic Transformation inJournal of fungi (Basel, Switzerland) · 2021Review
- Myristate can be used as a carbon and energy source for the asymbiotic growth of arbuscular mycorrhizal fungi.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- N-Myristoyltransferase as a Glycine and Lysine Myristoyltransferase in Cancer, Immunity, and Infections.ACS chemical biology · 2020Review
- Multiple Applications of a Transient CRISPR-Cas9 Coupled with Electroporation (TRACE) System in theGenetics · 2018Article
- Functional Characterization of Cryptococcal Genes: Then and Now.Frontiers in microbiology · 2018Review
- Virulence Factors as Targets for Anticryptococcal Therapy.Journal of fungi (Basel, Switzerland) · 2016Review
- Design, Synthesis and Antifungal Activity of Novel Benzofuran-Triazole Hybrids.Molecules (Basel, Switzerland) · 2016Article
- All about that fat: Lipid modification of proteins in Cryptococcus neoformans.Journal of microbiology (Seoul, Korea) · 2016Review
- The Mechanistic Targets of Antifungal Agents: An Overview.Mini reviews in medicinal chemistry · 2016Review
- Virulence-Associated Enzymes of Cryptococcus neoformans.Eukaryotic cell · 2015Review
- N-myristoyltransferase is a cell wall target in Aspergillus fumigatus.ACS chemical biology · 2015Article
- New technology and resources for cryptococcal research.Fungal genetics and biology : FG & B · 2015Review
- Generation of stable mutants and targeted gene deletion strains in Cryptococcus neoformans through electroporation.Medical mycology · 2015Article
- Peptidomimetic inhibitors of N-myristoyltransferase from human malaria and leishmaniasis parasites.Organic & biomolecular chemistry · 2014Article
- A target repurposing approach identifies N-myristoyltransferase as a new candidate drug target in filarial nematodes.PLoS neglected tropical diseases · 2014Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
Cryptococcus neoformans is a major cause of systemic fungal infection in immunocompromised patients. Myristoyl-CoA:protein N-myristoyltransferase (Nmt) catalyzes the transfer of myristate (C14:0) from myristoyl-CoA to the N-terminal glycine of a subset of cellular proteins produced during vegetative growth of C. neoformans. A Gly487-->Asp mutation was introduced into C. neoformans NMT by targeted gene replacement. The resulting strains are temperature-sensitive myristic acid auxotrophs. They are killed at 37 degrees C when placed in medium lacking myristate and, in an immunosuppressed animal model of cryptococcal meningitis, are completely eliminated from the subarachnoid space within 12 days of initial infection. C. neoformans and human Nmts exhibit differences in their peptide substrate specificities. These differences can be exploited to develop a new class of fungicidal drugs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.