ArticleProceedings of the National Academy of Sciences of the United States of America1994
Molecular cloning of a myosin I beta isozyme that may mediate adaptation by hair cells of the bullfrog's internal ear.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 40 citations in OpenAlex.
- Distribution of frequencies of spontaneous oscillations in hair cells of the bullfrog sacculus.Biophysical journal · 2009Article
- Spontaneous oscillation by hair bundles of the bullfrog's sacculus.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2003Article
- Calmodulin binding to recombinant myosin-1c and myosin-1c IQ peptides.BMC biochemistry · 2002Article
- Myosin-1c interacts with hair-cell receptors through its calmodulin-binding IQ domains.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2002Article
- Putting ion channels to work: mechanoelectrical transduction, adaptation, and amplification by hair cells.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Regulation of free Ca2+ concentration in hair-cell stereocilia.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1998Article
- Myosin VIIA is required for aminoglycoside accumulation in cochlear hair cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1997Article
- Unconventional myosins in inner-ear sensory epithelia.The Journal of cell biology · 1997Article
- ATPase activity of myosin in hair bundles of the bullfrog's sacculus.Biophysical journal · 1997Article
- Calmodulin controls adaptation of mechanoelectrical transduction by hair cells of the bullfrog's sacculus.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- A myosin family reunion.Journal of muscle research and cell motility · 1996Review
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The internal ear's sensory receptor, or hair cell, responds when stimuli deflect its mechanoreceptive hair bundle. As a hair cell adapts to sustained stimulation, mechanical adjustments within the bundle reset its position of sensitivity. Because several lines of experimentation suggest that a form of myosin I mediates adaptation, we endeavored to clone cDNAs encoding this motor molecule. By using degenerate oligonucleotide primers based upon the deduced amino acid sequence for mammalian myosin I beta, we performed reverse transcription and polymerase chain reactions (PCRs) to produce a candidate cDNA from polyadenylylated mRNA isolated from the frog's brain. The resultant product was used to probe a cDNA library, from which were isolated clones encoding an approximately 119-kDa isozyme of myosin I beta. PCR amplification disclosed the presence of mRNA encoding the same isozyme in tissue from the bullfrog's sacculus, an organ of the internal ear. When expressed as a bacterial fusion protein, a domain from the tail region of this form of myosin I was recognized by monoclonal antibodies that react with myosin I in hair bundles. This cloned approximately 119-kDa isozyme of myosin I is accordingly a candidate to be the motor molecule responsible for the adaptation of mechanoelectrical transduction by hair cells.
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