ArticleProceedings of the National Academy of Sciences of the United States of America1994
Identification of a 50-kDa systemin-binding protein in tomato plasma membranes having Kex2p-like properties.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed.
- A Bifunctional Synthetic Peptide With Antimicrobial and Plant Elicitation Properties That Protect Tomato Plants From Bacterial and Fungal Infections.Frontiers in plant science · 2021Article
- Toward Engineering the Mannose 6-Phosphate Elaboration Pathway in Plants for Enzyme Replacement Therapy of Lysosomal Storage Disorders.Journal of clinical medicine · 2019Article
- Tomato PEPR1 ORTHOLOG RECEPTOR-LIKE KINASE1 Regulates Responses to Systemin, Necrotrophic Fungi, and Insect Herbivory.The Plant cell · 2018Article
- Subtilisin-like proteases in plant defence: the past, the present and beyond.Molecular plant pathology · 2018Review
- Sugarcane Serine Peptidase Inhibitors, Serine Peptidases, and Clp Protease System Subunits Associated with Sugarcane Borer (Diatraea saccharalis) Herbivory and Wounding.International journal of molecular sciences · 2016Article
- RevisitingFrontiers in plant science · 2016Article
- Biochemical properties of the matrix metalloproteinase NtMMP1 from Nicotiana tabacum cv. BY-2 suspension cells.Planta · 2010Article
- The tomato brassinosteroid receptor BRI1 increases binding of systemin to tobacco plasma membranes, but is not involved in systemin signaling.Plant molecular biology · 2009Article
- Receptor-mediated signalling in plants: molecular patterns and programmes.Journal of experimental botany · 2009Review
- Tomato BRI1 and systemin wound signalling.Plant signaling & behavior · 2008Article
- Review
- Purification and characterization of serine proteases that exhibit caspase-like activity and are associated with programmed cell death in Avena sativa.The Plant cell · 2004Article
- An insect peptide engineered into the tomato prosystemin gene is released in transgenic tobacco plants and exerts biological activity.Plant molecular biology · 2003Article
- Ara12 subtilisin-like protease from Arabidopsis thaliana: purification, substrate specificity and tissue localization.The Biochemical journal · 2003Article
- Polypeptide hormones.The Plant cell · 2002Review
- RALF, a 5-kDa ubiquitous polypeptide in plants, arrests root growth and development.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- BRS1, a serine carboxypeptidase, regulates BRI1 signaling in Arabidopsis thaliana.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Plant proteolytic enzymes: possible roles during programmed cell death.Plant molecular biology · 2000Review
- Exploiting secondary growth in Arabidopsis. Construction of xylem and bark cDNA libraries and cloning of three xylem endopeptidases.Plant physiology · 2000Article
- A subtilisin-like serine protease involved in the regulation of stomatal density and distribution in Arabidopsis thaliana.Genes & development · 2000Article
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Authors and funding
2 authors.
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Abstract
A protein of 50-kDa (SBP50) was identified in plasma membranes of tomato leaves which resembles proteases of the family of Kex2p-like prohormone convertases. To our knowledge, proteases of this class have not been reported in plants previously. A biotinylated derivative of systemin, the 18-aa polypeptide inducer of proteinase inhibitors in tomato and potato leaves, was bound by SBP50 with high specificity. When a systemin derivative was labeled with biotin at residue 8 and with [35S]methionine at position 15, the biotin moiety but not the radioactive label was bound by SBP50. At least 4 aa from the C terminus that included [35S]methionine were missing, indicating that proteolytic cleavage had occurred. Whereas residues in systemin most important for binding SBP50 appear to be located in the N-terminal half of the molecule, amino acids crucial for proteinase inhibitor induction are located within the C terminus. The residues important for binding include a cleavage site for furin, a member of the family of Kex2p-like prohormone-processing enzymes. Processing of systemin at the predicted furin cleavage site was confirmed in vitro. An antiserum against a Kex2p-like protease from Drosophila inhibited binding of biotinylsystemin to SBP50 and recognized a protein of about 60 kDa in Western blot analyses of tomato plasma membrane proteins. The data suggest a possible role for a membrane bound, furin-like protease in the mechanism of defense gene signaling by systemin.
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