ArticleHuman genetics1994
New sites of methylcytosine-rich DNA detected on metaphase chromosomes.
Article in Human genetics, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed.
- Article
- Infrared nanospectroscopic mapping of a single metaphase chromosome.Nucleic acids research · 2019Article
- Differential hypomethylation of the repetitiveComparative cytogenetics · 2018Article
- Genome-wide 5-hydroxymethylcytosine patterns in human spermatogenesis are associated with semen quality.Oncotarget · 2017Article
- Epigenetic modifications in sex heterochromatin of vole rodents.Chromosoma · 2015Article
- Immunofluorescent staining reveals hypermethylation of microchromosomes in the central bearded dragon, Pogona vitticeps.Molecular cytogenetics · 2015Article
- Methylated-rich regions and tandem repeat arrays along the chromosome complement of Colpodium versicolor (Stev.) Schmalh.Protoplasma · 2009Article
- Histone modifications and nuclear architecture: a review.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2008Review
- Article
- Epigenetic control of nuclear architecture.Cellular and molecular life sciences : CMLS · 2007Review
- Engineering a high-affinity methyl-CpG-binding protein.Nucleic acids research · 2006Article
- Short interspersed transposable elements (SINEs) are excluded from imprinted regions in the human genome.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Active repression of methylated genes by the chromosomal protein MBD1.Molecular and cellular biology · 2000Article
- Common methylation characteristics of sex chromosomes in somatic and germ cells from mouse, lemur and human.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2000Article
- Identifying 5-methylcytosine and related modifications in DNA genomes.Nucleic acids research · 1998Review
- DNA methylation of the extraembryonic tissues: an in situ study on human metaphase chromosomes.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 1998Article
- DNA methylation of the X chromosomes of the human female: an in situ semi-quantitative analysis.Chromosoma · 1996Article
- Distribution of 5-methylcytosine-rich regions in the metaphase chromosomes of Vicia faba.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 1996Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In situ immunofluorescence detection of antibodies against 5-methylcytosine on metaphase chromosomes prepared by a new procedure allows the display of new 5-methylcytosine-rich sites as compared to previously published methods. In short-term culture lymphocytes, the immunofluorescent signals give a recurrent pattern in which four types of binding sites can be distinguished. Type I sites are the secondary constrictions and a few juxtacentromeric regions, type II sites correspond to T-bands. Both types I and II sites emit a strong fluorescence. Type III sites form an R-band pattern and emit a weaker fluorescence. Type IV sites are the short arms of acrocentrics, they emit strong but polymorphic signals. The results obtained from control experiments suggest that the pattern observed is rather the expression of an uneven distribution of 5-methylcytosine-rich sites than a consequence of the various treatments used. In a lymphoblastoid cell line known to have a reduced 5-methylcytosine content, it was possible to demonstrate a heterogeneous hypomethylation among chromosome structures, principally involving type I sites. The method opens the possibility of studying in situ on chromosomes, regional variations of methylation in pathological conditions.
Indexed as
Identifiers
7989044What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.