ArticleHuman genetics1994
Presence of chromosomal mosaicism in abnormal preimplantation embryos detected by fluorescence in situ hybridisation.
Article in Human genetics, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Preimplantation chromosomal mosaics, chimaeras and confined placental mosaicism.Reproduction & fertility · 2022Review
- Let the data do the talking: the need to consider mosaicism during embryo selection.Fertility and sterility · 2021Review
- Lack of evidence to support recommendation for prenatal uniparental disomy (UPD) analysis following mosaic embryo transfer.Genetics in medicine : official journal of the American College of Medical Genetics · 2021Article
- The parental origin correlates with the karyotype of human embryos developing from tripronuclear zygotes.Clinical and experimental reproductive medicine · 2015Article
- Mosaic variegated aneuploidy in mouse BubR1 deficient embryos and pregnancy loss in human.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2014Article
- Altered cleavage patterns in human tripronuclear embryos and their association to fertilization method: a time-lapse study.Journal of assisted reproduction and genetics · 2014Article
- Preimplantation genetic diagnosis for aneuploidy and translocations using array comparative genomic hybridization.Current genomics · 2012Article
- What next for preimplantation genetic screening? A polar body approach!Human reproduction (Oxford, England) · 2010Article
- Detection of embryo sex chromosome by dual color fluorescent in-situ hybridization.Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban · 2003Article
- Different probe combinations for assessment of postzygotic chromosomal imbalances in human embryos.Journal of assisted reproduction and genetics · 2002Article
- Preimplantation genetic diagnosis of aneuploidy: were we looking at the wrong chromosomes?Journal of assisted reproduction and genetics · 1999Article
- Influence of mosaicism on sexing of human preembryos detected by the polymerase chain reaction.Journal of assisted reproduction and genetics · 1996Article
- Clinical application of FISH for sex determination of embryos in preimplantation diagnosis of X-linked diseases.Journal of assisted reproduction and genetics · 1996Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
The extent of chromosomal mosaicism in human preimplantation embryos was examined using an improved procedure for the preparation and spreading of interphase nuclei for use in fluorescence in situ hybridisation, allowing the analysis of every nucleus within an embryo. One cell showed no hybridisation signals in only three of the 38 embryos that were included in this study, i.e. the hybridisation efficiency per successfully spread nucleus was 99% (197/200). Double-target in situ hybridisation analyses with X- and Y-chromosome-specific probes was performed to analyse nine embryos resulting from normal fertilisation, 22 polypronucleate embryos and seven cleavage-stage embryos where no (apronucleate) or only one pronucleus (monopronucleate) was observed. We also analysed autosomes 1 and 7 by double-target in situ hybridisation in the nuclei of two apronucleate, one monopronucleate and four polypronucleate embryos. All nine embryos that resulted from normal fertilisation were uniformly XY or XX. None of the apronucleate or monopronucleate embryos was haploid: three were diploid, one was triploid and three were mosaic. Fertilisation was detected by the presence of a Y-specific signal in four of these embryos. Of the polypronucleate embryos, two were diploid, two were triploid and 18 were mosaic for the sex chromosomes and/or autosomes 1 and 7. These results demonstrate that fertilisation sometimes occurs in monopronucleate embryos and that chromosomal mosaicism can be detected with high efficiency in apronucleate, monopronucleate and polypronucleate human embryos using fluorescence in situ hybridisation.
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