ArticleNucleic acids research1994
The presence of both negative and positive elements in the 5'-flanking sequence of the rat Na,K-ATPase alpha 3 subunit gene are required for brain expression in transgenic mice.
Article in Nucleic acids research, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 48 citations in OpenAlex.
- Transcriptional regulators of Na,K-ATPase subunits.Frontiers in cell and developmental biology · 2015Review
- The expression of the human neuronal alpha3 Na+,K+-ATPase subunit gene is regulated by the activity of the Sp1 and NF-Y transcription factors.The Biochemical journal · 2005Article
- The neuron-restrictive silencer element-neuron-restrictive silencer factor system regulates basal and endothelin 1-inducible atrial natriuretic peptide gene expression in ventricular myocytes.Molecular and cellular biology · 2001Article
- Expression of brain-derived neurotrophic factor in cortical neurons is regulated by striatal target area.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2001Article
- Transcriptional repression by neuron-restrictive silencer factor is mediated via the Sin3-histone deacetylase complex.Molecular and cellular biology · 2000Article
- Transcriptional regulation of the GluR2 gene: neural-specific expression, multiple promoters, and regulatory elements.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1998Article
- Neuronal expression of zinc finger transcription factor REST/NRSF/XBR gene.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1998Article
- Na,K-ATPase mRNA levels and plaque load in Alzheimer's disease.Journal of molecular neuroscience : MN · 1997Article
- Tissue-specific expression of the L1 cell adhesion molecule is modulated by the neural restrictive silencer element.The Journal of cell biology · 1997Article
- The neuron-restrictive silencer element: a dual enhancer/silencer crucial for patterned expression of a nicotinic receptor gene in the brain.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Polymorphism and structure of the gene coding for the alpha 1 subunit of the Artemia franciscana Na/K-ATPase.The Biochemical journal · 1997Article
- Identification of cis-acting sequences in the promoter of the herpes simplex virus type 1 latency-associated transcripts required for activation by nerve growth factor and sodium butyrate in PC12 cells.Journal of virology · 1996Article
- Identification of potential target genes for the neuron-restrictive silencer factor.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- Two alternative promoters direct neuron-specific expression of the rat microtubule-associated protein 1B gene.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1996Article
- Ubiquitous and neuronal DNA-binding proteins interact with a negative regulatory element of the human hypoxanthine phosphoribosyltransferase gene.Molecular and cellular biology · 1995Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The Na,K-ATPase is an integral plasma membrane protein consisting of alpha and beta subunits, each of which has discrete isoforms expressed in a tissue-specific manner. Of the three functional alpha isoform genes, the one encoding the alpha 3 isoform is the most tissue-restricted in its expression, being found primarily in the brain. To identify regions of the alpha 3 isoform gene that are involved in directing expression in the brain, a 1.6 kb 5'-flanking sequence was attached to a reporter gene, chloramphenicol acetyltransferase (CAT). The alpha 3-CAT chimeric gene construct was microinjected into fertilized mouse eggs, and transgenic mice were produced. Analysis of adult transgenic mice from different lines revealed that the transgene is expressed primarily in the brain. To further delineate regions that are needed for conferring expression in this tissue, systematic deletions of the 5'-flanking sequence of the alpha 3-CAT fusion constructs were made and analyzed, again using transgenic mice. The results from these analyses indicate that DNA sequences required for mediating brain-specific expression of the alpha 3 isoform gene are present within 210 bp upstream of the transcription initiation site. alpha 3-CAT promoter constructs containing scanning mutations in this region were also assayed in transgenic mice. These studies have identified both a functional neural-restrictive silencer element as well as a positively acting cis element.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.