ArticleNucleic acids research1994
Identification of a 59 bp enhancer located at the first exon/intron boundary of the human O6-methylguanine DNA methyltransferase gene.
Article in Nucleic acids research, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 45 citations in OpenAlex.
- MGMT epimutations and risk of incident cancer of the colon, glioblastoma multiforme, and diffuse large B cell lymphomas.Clinical epigenetics · 2025Article
- Enhancing Temozolomide (TMZ) chemosensitivity using CRISPR-dCas9-mediated downregulation of OJournal of neuro-oncology · 2024Article
- Association betweenCells · 2023Article
- Discrimination between 34 of 36 Possible Combinations of Three C>T SNP Genotypes in theInternational journal of molecular sciences · 2021Article
- Genomic Space ofInternational journal of molecular sciences · 2021Article
- MGMT promoter methylation level in newly diagnosed low-grade glioma is a predictor of hypermutation at recurrence.Neuro-oncology · 2020Article
- Weak MGMT gene promoter methylation confers a clinically significant survival benefit in patients with newly diagnosed glioblastoma: a retrospective cohort study.Journal of neuro-oncology · 2020Article
- MRI to MGMT: predicting methylation status in glioblastoma patients using convolutional recurrent neural networks.Pacific Symposium on Biocomputing. Pacific Symposium on Biocomputing · 2018Article
- SNP rs16906252C>T Is an Expression and Methylation Quantitative Trait Locus Associated with an Increased Risk of Developing MGMT-Methylated Colorectal Cancer.Clinical cancer research : an official journal of the American Association for Cancer Research · 2016Article
- Evolution of DNA repair defects during malignant progression of low-grade gliomas after temozolomide treatment.Acta neuropathologica · 2015Article
- Response of primary glioblastoma cells to therapy is patient specific and independent of cancer stem cell phenotype.Neuro-oncology · 2014Article
- Influence of promoter/enhancer region haplotypes on MGMT transcriptional regulation: a potential biomarker for human sensitivity to alkylating agents.Carcinogenesis · 2014Article
- Combined analysis of O6-methylguanine-DNA methyltransferase protein expression and promoter methylation provides optimized prognostication of glioblastoma outcome.Neuro-oncology · 2013Article
- Prognosis of oligodendroglial tumor with ring enhancement showing central necrotic portion.Journal of neuro-oncology · 2011Article
- The A/G allele of rs16906252 predicts for MGMT methylation and is selectively silenced in premalignant lesions from smokers and in lung adenocarcinomas.Clinical cancer research : an official journal of the American Association for Cancer Research · 2011Article
- Aberrant MGMT (O6-methylguanine-DNA methyltransferase) promoter methylation in choroid plexus tumors.Journal of neuro-oncology · 2009Article
- Article
- CpG methylation of MGMT and hMLH1 promoter in hepatocellular carcinoma associated with hepatitis viral infection.British journal of cancer · 2003Article
- Frequent promoter hypermethylation of the O6-Methylguanine-DNA Methyltransferase (MGMT) gene in testicular cancer.Oncogene · 2002Article
- Methylation of discrete regions of the O6-methylguanine DNA methyltransferase (MGMT) CpG island is associated with heterochromatinization of the MGMT transcription start site and silencing of the gene.Molecular and cellular biology · 1997Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The DNA repair enzyme, O6-methylguanine DNA methyltransferase (MGMT) is responsible for repair of damage induced by alkylating agents that produce adducts at O6-guanine in DNA. Although the MGMT gene promoter has housekeeping gene promoter characteristics, unlike these genes which are expressed at a constant level, MGMT transcriptional activity varies between cell types. During an attempt to identify regions of the MGMT regulatory sequence sensitive to variations in transcription factors between cell types, we have identified a 59 bp enhancer which is required for efficient MGMT promoter function. This fragment produced increased transcriptional activity in reporter gene constructs containing either the MGMT or UMP-synthase promoter when transfected into either of two cell lines; it seems therefore that this enhancer may interact with relatively common trans-acting factors. Functional activity is only detected when the enhancer is in 'cis' with respect to the promoter, suggesting that complexes are formed between proteins bound to the enhancer and promoter sequences. We propose that the enhancer-binding protein may be a novel transcription factor since there are no obvious consensus sequences within the 59 bp sequence for known DNA-binding proteins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.