ArticleNucleic acids research1994
Target-specific arrest of mRNA translation by antisense 2'-O-alkyloligoribonucleotides.
Article in Nucleic acids research, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 61 citations in OpenAlex.
- The Coming Age of Antisense Oligos for the Treatment of Hepatic Ischemia/Reperfusion (IRI) and Other Liver Disorders: Role of Oxidative Stress and Potential Antioxidant Effect.Antioxidants (Basel, Switzerland) · 2024Review
- RNA Therapeutics for Improving CAR T-cell Safety and Efficacy.Cancer research · 2023Review
- Targeting 3' and 5' untranslated regions with antisense oligonucleotides to stabilize frataxin mRNA and increase protein expression.Nucleic acids research · 2021Article
- CUG initiation and frameshifting enable production of dipeptide repeat proteins from ALS/FTD C9ORF72 transcripts.Nature communications · 2018Article
- Inhibition of Non-ATG Translational Events in Cells via Covalent Small Molecules Targeting RNA.Journal of the American Chemical Society · 2015Article
- Enhancement of the click chemistry for the inverse Diels Alder technology by functionalization of amide-based monomers.International journal of medical sciences · 2011Article
- Extension of the PNA world by functionalized PNA monomers eligible candidates for inverse Diels Alder Click Chemistry.International journal of medical sciences · 2010Article
- Chemical synthesis of LNA-2-thiouridine and its influence on stability and selectivity of oligonucleotide binding to RNA.Biochemistry · 2009Article
- RNA bandages for photoregulating in vitro protein synthesis.Bioorganic & medicinal chemistry letters · 2008Article
- A dormant internal ribosome entry site controls translation of feline immunodeficiency virus.Journal of virology · 2008Article
- Psoralen photo-crosslinked mRNA-puromycin conjugates: a novel template for the rapid and facile preparation of mRNA-protein fusions.Nucleic acids research · 2000Article
- Effects of RNA secondary structure on cellular antisense activity.Nucleic acids research · 2000Article
- Inhibition of in vitro and ex vivo translation by a transplatin-modified oligo(2'-O-methylribonucleotide) directed against the HIV-1 gag-pol frameshift signal.Nucleic acids research · 2000Article
- Translational activation of uncapped mRNAs by the central part of human eIF4G is 5' end-dependent.RNA (New York, N.Y.) · 1998Article
- Advantages of 2'-O-methyl oligoribonucleotide probes for detecting RNA targets.Nucleic acids research · 1998Article
- Translation in plants--rules and exceptions.Plant molecular biology · 1996Review
- Antisense properties of duplex- and triplex-forming PNAs.Nucleic acids research · 1996Article
- A synthetic, chemically modified ribozyme eliminates amelogenin, the major translation product in developing mouse enamel in vivo.The EMBO journal · 1995Article
- RNase H is responsible for the non-specific inhibition of in vitro translation by 2'-O-alkyl chimeric oligonucleotides: high affinity or selectivity, a dilemma to design antisense oligomers.Nucleic acids research · 1995Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We describe a novel experimental approach to investigate mRNA translation. Antisense 2'-O-allyl oligoribonucleotides (oligos) efficiently arrest translation of targeted mRNAs in rabbit reticulocyte lysate and wheat germ extract while displaying minimal non-specific effects on translation. Oligo/mRNA-hybrids positioned anywhere within the 5' UTR or the first approximately 20 nucleotides of the open reading frame block cap-dependent translation initiation with high specificity. The thermodynamic stability of hybrids between 2'-O-alkyl oligos and RNA permits translational inhibition with oligos as short as 10 nucleotides. This inhibition is independent of RNase H cleavage or modifications which render the mRNA untranslatable. We show that 2'-O-alkyl oligos can also be employed to interfere with cap-independent internal initiation of translation and to arrest translation elongation. The latter is accomplished by UV-crosslinking of psoralen-tagged 2'-O-methyloligoribonucleotides to the mRNA within the open reading frame. The utility of 2'-O-alkyloligoribonucleotides to arrest translation from defined positions within an mRNA provides new approaches to investigate mRNA translation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.