ArticleJournal of virology1995
Defective RNA replication by poliovirus mutants deficient in 2A protease cleavage activity.
Article in Journal of virology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 45 citations in OpenAlex.
- Picornavirus Evolution: Genomes Encoding Multiple 2AViruses · 2024Article
- Article
- The mammalian host protein DAP5 facilitates the initial round of translation of Coxsackievirus B3 RNA.The Journal of biological chemistry · 2019Article
- Structural view of the 2A protease from human rhinovirus C15.Acta crystallographica. Section F, Structural biology communications · 2018Article
- Adaptive evolution and demographic history contribute to the divergent population genetic structure ofEvolutionary applications · 2017Article
- Deubiquitinase function of arterivirus papain-like protease 2 suppresses the innate immune response in infected host cells.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- The multifaceted poliovirus 2A protease: regulation of gene expression by picornavirus proteases.Journal of biomedicine & biotechnology · 2011Article
- RIG-I is cleaved during picornavirus infection.Virology · 2009Article
- The porcine reproductive and respiratory syndrome virus nsp2 cysteine protease domain possesses both trans- and cis-cleavage activities.Journal of virology · 2009Article
- MDA-5 is cleaved in poliovirus-infected cells.Journal of virology · 2007Article
- Blockade of the poliovirus-induced cytopathic effect in neural cells by monoclonal antibody against poliovirus or the human poliovirus receptor.Journal of virology · 2005Article
- Cumulative mutations in the genome of Echovirus 6 during establishment of a chronic infection in precursors of glial cells.Virus genes · 2005Article
- Strand-specific RNA synthesis defects in a poliovirus with a mutation in protein 3A.Journal of virology · 2003Article
- A stable HeLa cell line that inducibly expresses poliovirus 2A(pro): effects on cellular and viral gene expression.Journal of virology · 2000Article
- Cleavage of eukaryotic translation initiation factor 4G by exogenously added hybrid proteins containing poliovirus 2Apro in HeLa cells: effects on gene expression.Molecular and cellular biology · 1999Article
- A protein linkage map of the P2 nonstructural proteins of poliovirus.Journal of virology · 1998Article
- Protein 2A is not required for Theiler's virus replication.Journal of virology · 1997Article
- Comparison of the replication of positive-stranded RNA viruses of plants and animals.Advances in virus research · 1996Review
- Viral cysteine proteinases.Perspectives in drug discovery and design : PD3 · 1996Article
- Analysis of picornavirus 2A(pro) proteins: separation of proteinase from translation and replication functions.Journal of virology · 1995Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
2A protease (2Apro) catalyzes the initial cleavage of the poliovirus polyprotein which separates the P1 structural protein precursor from the P2-P3 nonstructural protein precursor. In addition, 2Apro indirectly induces cleavage of the p220 component of eukaryotic initiation factor 4F, which is thought to contribute to the specific inhibition of host cell protein synthesis observed in virus-infected HeLa cells. However, it is unclear whether the trans function of 2Apro which induces host cell shutoff is essential or merely facilitates efficient poliovirus replication. In this study, three point mutations in 2Apro (D38E, Y88L, and Y89L [S. F. Yu and R. E. Lloyd, Virology 182:615-625, 1991]) which cause specific loss of trans but not cis cleavage function were independently introduced into the full-length poliovirus cDNA. In addition, mutations which caused only partial loss of both cis and trans cleavage activities (Y88S) or resulted in a wild-type phenotype (Y88F) were individually introduced. When each of these mutant poliovirus cDNAs was transcribed and translated in vitro, normal proteolytic processing of the viral polyprotein was observed, and p220 was not cleaved in those reactions containing proteases defective in trans function, as expected. Surprisingly, Northern (RNA) blot analysis and reverse transcriptase-PCRs performed after transfection of COS-7 or HeLa cells with these viral RNAs revealed that Y88S and Y88L RNAs replicated at only very low levels. RNA replication could not be detected at all in cells transfected with D38E and Y89L RNAs. Taken together, the results suggest a correlation between the function of 2Apro and productive poliovirus RNA replication in vivo that may be independent of the ability to cause p220 cleavage.
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