ArticleArchives of dermatological research1994
Human eosinophils stimulate DNA synthesis and matrix production in dermal fibroblasts.
Article in Archives of dermatological research, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
12 citing papers in PubMed, 55 citations in OpenAlex.
- Could the Epigenetics of Eosinophils in Asthma and Allergy Solve Parts of the Puzzle?International journal of molecular sciences · 2021Review
- The endometrial immune environment of women with endometriosis.Human reproduction update · 2019Review
- The Biology of Eosinophils and Their Role in Asthma.Frontiers in medicine · 2017Review
- Host protective roles of type 2 immunity: parasite killing and tissue repair, flip sides of the same coin.Seminars in immunology · 2014Review
- Scleroderma-like fibrosing disorders.Rheumatic diseases clinics of North America · 2008Review
- Eosinophil infiltration and degranulation in oesophageal mucosa from adult patients with eosinophilic oesophagitis: a retrospective and comparative study on pathological biopsy.Journal of clinical pathology · 2006Article
- The effect of eosinophils on collagen gel contraction and implications for tissue remodelling.Clinical and experimental immunology · 2004Article
- Dose-dependent impairment of collagen deposition by topical granulocyte-macrophage colony-stimulating factor in human experimental wounds.Annals of surgery · 2002Article
- Eosinophil granule-derived major basic protein induces IL-8 expression in human intestinal myofibroblasts.Clinical and experimental immunology · 2000Article
- Degranulating eosinophils in human endometriosis.The American journal of pathology · 2000Article
- Human eosinophils regulate human lung- and skin-derived fibroblast properties in vitro: a role for transforming growth factor beta (TGF-beta).Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Critical roles for IL-4, IL-5, and eosinophils in chronic skin allograft rejection.The Journal of clinical investigation · 1999Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fibrosis is frequently found in diseases exhibiting tissue eosinophilia, such as some parasitic worm infections, eosinophilia-myalgia syndrome, and eosinophilic fasciitis. Previously, eosinophil extracts have been shown to induce proliferation in neonatal foreskin fibroblasts in vitro. To determine if living eosinophils can induce synthesis of DNA and components of the extracellular matrix in dermal fibroblasts, we cultured purified human eosinophils for 2 or 7 days in the presence of the eosinophil-active cytokines granulocyte/macrophage colony stimulating factor, interleukin-3, or interleukin-5, and added eosinophil-conditioned medium to cultures of dermal fibroblasts. Using flow cytometry, we found that eosinophil-conditioned medium increased by two-fold the percentage of fibroblasts in S-phase. This stimulation of fibroblast DNA synthesis was corroborated using a standard tritiated thymidine assay and the two methods were shown to correlate well with each other. Eosinophil-conditioned medium stimulation of DNA synthesis was dose dependent and conditioned medium from eosinophils treated with any one of the three cytokines induced increased DNA synthesis. Treatment of fibroblasts with cytokines alone did not induce enhanced DNA synthesis. Eosinophil-conditioned medium also affected fibroblast matrix production. Eosinophil-conditioned medium induced a two-fold increase in soluble and cell-associated fibroblast glycosaminoglycan production and a 76% increase in collagen production. These observations support the concept that eosinophils may be active contributors to the pathophysiology of eosinophil-associated fibrotic disease.
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Registered trials
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