ArticleThorax1994
Heterogeneous expression and polymorphic genotype of glutathione S-transferases in human lung.
Article in Thorax, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers, 1 of them a synthesis that pooled it.
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Who cites it
27 citing papers in PubMed, 1 synthesis or guideline pooled it, 82 citations in OpenAlex.
- Pooled it
- Article
- In vitro airway models from mice, rhesus macaques, and humans maintain species differences in xenobiotic metabolism and cellular responses to naphthalene.American journal of physiology. Lung cellular and molecular physiology · 2022Article
- Article
- Variants in genes coding for glutathione S-transferases and asthma outcomes in children.Pharmacogenomics · 2018Article
- GSTP1 Ile105Val polymorphism might be associated with the risk of radiation pneumonitis among lung cancer patients in Chinese population: A prospective study.Journal of Cancer · 2018Article
- Polymorphisms and Protein Expressions of Glutathione S-Transferase M1 and T1 in Non-Small Cell Lung Cancer.Turkish journal of pharmaceutical sciences · 2017Article
- Biomarkers in Occupational Asthma.Current allergy and asthma reports · 2016Review
- Article
- Genetic determinants of chronic obstructive pulmonary disease in South Indian male smokers.PloS one · 2014Article
- Genetic variants in antioxidant genes are associated with diisocyanate-induced asthma.Toxicological sciences : an official journal of the Society of Toxicology · 2012Article
- Role of GSTM1 in resistance to lung inflammation.Free radical biology & medicine · 2012Review
- Glutathione-S-transferase M1 regulation of diesel exhaust particle-induced pro-inflammatory mediator expression in normal human bronchial epithelial cells.Particle and fibre toxicology · 2012Article
- GSTM1 modulation of IL-8 expression in human bronchial epithelial cells exposed to ozone.Free radical biology & medicine · 2011Article
- Glutathione S-transferase copy number variation alters lung gene expression.The European respiratory journal · 2011Article
- Smoking-Related Gene Expression in Laser Capture-Microdissected Human Lung.Clinical cancer research : an official journal of the American Association for Cancer Research · 2009Article
- Glutathione-S-transferases in lung and sputum specimens, effects of smoking and COPD severity.Respiratory research · 2008Article
- Chronic obstructive pulmonary disease. 1: Susceptibility factors for COPD the genotype-environment interaction.Thorax · 2002Review
- Article
- Glutathione S-transferase P1 (GSTP1) polymorphism in patients with chronic obstructive pulmonary disease.Thorax · 1999Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundGlutathione S-transferases (GSTs) are involved in the detoxification of xenobiotics by conjugation with glutathione. One of the mu class genes of this superfamily of enzymes, GSTM1, is polymorphic because of a partial gene deletion. This results in a failure to express GSTM1 in approximately 50% of individuals. Several studies have linked GSTM1 null status to an increased risk of lung carcinoma. This study investigated the expression and distribution of GST isoenzymes in human lung, and developed a polymerase chain reaction (PCR) assay which would allow genotyping of archival, paraffin embedded lung tissue.
methodsDistribution was examined using a panel of polyclonal anti-GST antibodies for immunohistochemistry in normal tissue of 21 tumour-bearing lungs. DNA for PCR was extracted from paraffin blocks and a control group of 350 blood lysates. As a positive control each assay amplified part of GSTM4, a mu class gene which is not polymorphic but which shows strong sequence homology to GSTM1. The presence of GST in bronchoalveolar lavage fluid was sought by Western analysis.
resultsProximal airways contained pi class GST, alpha class GST, and mu class GST with expression concentrated in the brush border. In distal airspaces no alpha GST was expressed but pi GST and mu GST were present in alveolar cells and also alveolar macrophages. Pi class GST was present in bronchoalveolar lavage fluid. The PCR assay enabled genotypic determination using DNA extracted from archival material. Of the control group 56% were null at the GSTM1 locus.
conclusionsThe distribution of GST isoenzymes in the lung is heterogeneous with an apparent decrease in GST in distal lung. Since GSTM1 status has already been associated with susceptibility to disease, the PCR assay developed will allow further studies of the relation between genotype and structural disorders in the lung using archival pathological material.
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