ArticleProceedings of the National Academy of Sciences of the United States of America1994
Transplantation of an oligodendrocyte cell line leading to extensive myelination.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 1 synthesis or guideline pooled it, 134 citations in OpenAlex.
- Cell replacement therapy with stem cells in multiple sclerosis, a systematic review.Human cell · 2024Pooled it
- Investigating the Protective Effects of a Citrus Flavonoid on the Retardation Morphogenesis of the Oligodendroglia-like Cell Line by Rnd2 Knockdown.Neurology international · 2023Article
- Pearls and Pitfalls of Isolating Rat OPCs for In Vitro Culture with Different Methods.Cellular and molecular neurobiology · 2023Article
- Development of a Chemical Cocktail That Rescues Mouse Brain Demyelination in a Cuprizone-Induced Model.Cells · 2022Article
- ABCA1/ApoE/HDL Signaling Pathway Facilitates Myelination and Oligodendrogenesis after Stroke.International journal of molecular sciences · 2020Article
- RhoA-inhibiting NSAIDs promote axonal myelination after spinal cord injury.Experimental neurology · 2011Article
- The myelin mutants as models to study myelin repair in the leukodystrophies.Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics · 2011Review
- Conditional Tet-regulated over-expression of Hoxa2 in CG4 cells increases their proliferation and delays their differentiation into oligodendrocyte-like cells expressing myelin basic protein.Cellular and molecular neurobiology · 2011Article
- Glial cell lines: an overview.Neurochemical research · 2010Review
- From stem cells to oligodendrocytes: prospects for brain therapy.Stem cell reviews · 2007Review
- Strategies for achieving and monitoring myelin repair.Journal of neurology · 2007Review
- Oligodendroglial progenitor cell therapy limits central neurological deficits in mice with metachromatic leukodystrophy.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2006Article
- Glial grafting for demyelinating disease.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2005Review
- Cell therapy in demyelinating diseases.NeuroRx : the journal of the American Society for Experimental NeuroTherapeutics · 2004Review
- Derivation and characterization of monkey embryonic stem cells.Reproductive biology and endocrinology : RB&E · 2004Review
- Remyelination, axonal sparing, and locomotor recovery following transplantation of glial-committed progenitor cells into the MHV model of multiple sclerosis.Experimental neurology · 2004Article
- Remyelination of the adult demyelinated mouse brain by grafted oligodendrocyte progenitors and the effect of B-104 cografts.Neurochemical research · 2001Article
- Neurotransplantation of magnetically labeled oligodendrocyte progenitors: magnetic resonance tracking of cell migration and myelination.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Temporal and quantitative expression of the myelin-associated lipids, ethanolamine plasmalogen, galactocerebroside, and sulfatide, in the differentiating CG-4 glial cell line.Neurochemical research · 1999Article
- Therapeutic strategies in multiple sclerosis. II. Long-term repair.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 1999Review
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
Oligodendrocytes, the myelin-forming cells of the central nervous system, can be generated from progenitor cell lines and assayed for their myelinating properties after transplantation. A growth-factor-dependent cell line of rat oligodendrocyte progenitors (CG4) was carried through 31-48 passages before being transplanted into normal newborn rat brain or the spinal cord of newborn myelin-deficient (md) rats. In md rat spinal cord, CG4 oligodendrocyte progenitors migrated up to 7 mm along the dorsal columns, where they divided and myelinated numerous axons 2 weeks after grafting. CG4 cells were transfected with the bacterial lacZ gene and selected for high beta-galactosidase expression. The cell migration and fate of these LacZ+ cells were analyzed after transplantation. In normal newborn brain, LacZ+ oligodendrocyte progenitors migrated along axonal tracts from the site of injection and integrated in the forming white matter. In md rats, extensive migration (up to 12 mm) was revealed by staining for beta-galactosidase activity of the intact spinal cord where many grafted cells had moved into the posterior columns. Similar migration and integration of grafted cells occurred in the spinal cord of normal myelinated rats and after a noninvasive grafting procedure. Thus, oligodendrocyte progenitors can maintain their ability to migrate and myelinate axons in vivo after multiple passages in vitro. Such progenitor cell lines can be used to study the molecular mechanisms underlying oligodendrocyte development and the repair of myelin in dysmyelinating diseases.
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